Appendix 1: Chilean Smicridea (S. ) specimens used in the study

Appendix 1: Chilean Smicridea (S. ) specimens used in the study. Region Site Elevation Latitude Longitude Date Taxon AN AN AN AN AN AN AN AN AN

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Appendix 1: Chilean Smicridea (S. ) specimens used in the study. Region Site

Elevation

Latitude

Longitude

Date

Taxon

AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN VC VC VC VC VC VC VC VC VC AN AN AN AN AN CR CR CR CR CR CR CR CR AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN

1652 m asl 1170 m asl 1110 m asl 1110 m asl 1110 m asl 1110 m asl 1110 m asl 1110 m asl 1110 m asl 1110 m asl 1110 m asl 1110 m asl 1110 m asl 1110 m asl 1110 m asl 1110 m asl 1110 m asl 1110 m asl 1110 m asl 181 m asl 181 m asl 181 m asl 181 m asl 181 m asl 181 m asl 181 m asl 181 m asl 181 m asl 917 m asl 1075 m asl 1075 m asl 1075 m asl 1075 m asl 180 m asl 180 m asl 180 m asl 180 m asl 180 m asl 180 m asl 180 m asl 180 m asl 330 m asl 330 m asl 330 m asl 330 m asl 330 m asl 330 m asl 490 m asl 490 m asl 490 m asl 490 m asl 490 m asl 490 m asl 490 m asl 490 m asl 490 m asl 490 m asl 490 m asl

33°49.419'S 33°49.890'S 34°18.183'S 34°18.183'S 34°18.183'S 34°18.183'S 34°18.183'S 34°18.183'S 34°18.183'S 34°18.183'S 34°18.183'S 34°18.183'S 34°18.183'S 34°18.183'S 34°18.183'S 34°18.183'S 34°18.183'S 34°18.183'S 34°18.183'S 35°11.110'S 35°11.110'S 35°11.110'S 35°11.110'S 35°11.110'S 35°11.110'S 35°11.110'S 35°11.110'S 35°11.110'S 35°27.583'S 35°28.578'S 35°28.578'S 35°28.578'S 35°28.578'S 35°50.013'S 35°50.013'S 35°50.013'S 35°50.013'S 35°50.013'S 35°50.013'S 35°50.013'S 35°50.013'S 35°55.164'S 35°55.164'S 35°55.164'S 35°55.164'S 35°55.164'S 35°55.164'S 35°56.583'S 35°56.583'S 35°56.583'S 35°56.583'S 35°56.583'S 35°56.583'S 35°56.583'S 35°56.583'S 35°56.583'S 35°56.583'S 35°56.583'S

70°07.300'W 70°07.008'W 70°27.054'W 70°27.054'W 70°27.054'W 70°27.054'W 70°27.054'W 70°27.054'W 70°27.054'W 70°27.054'W 70°27.054'W 70°27.054'W 70°27.054'W 70°27.054'W 70°27.054'W 70°27.054'S 70°27.054'S 70°27.054'S 70°27.054'S 71°23.716'W 71°23.716'W 71°23.716'W 71°23.716'W 71°23.716'W 71°23.716'W 71°23.716'W 71°23.716'W 71°23.716'W 71°01.705'W 70°59.662'W 70°59.662'W 70°59.662'W 70°59.662'W 72°30.602'W 72°30.602'W 72°30.602'W 72°30.602'W 72°30.602'W 72°30.602'W 72°30.602'W 72°30.602'W 71°22.948'W 71°22.948'W 71°22.948'W 71°22.948'W 71°22.948'W 71°22.948'W 72°05.061'W 72°05.061'W 72°05.061'W 72°05.061'W 72°05.061'W 72°05.061'W 72°05.061'W 72°05.061'W 72°05.061'W 72°05.061'W 72°05.061'W

23-Jan-08 23-Jan-08 24-Jan-08 24-Jan-08 24-Jan-08 24-Jan-08 24-Jan-08 24-Jan-08 24-Jan-08 24-Jan-08 24-Jan-08 24-Jan-08 24-Jan-08 24-Jan-08 24-Jan-08 24-Jan-08 24-Jan-08 24-Jan-08 24-Jan-08 19-Feb-08 19-Feb-08 19-Feb-08 19-Feb-08 19-Feb-08 19-Feb-08 19-Feb-08 19-Feb-08 19-Feb-08 21-Feb-08 20-Feb-08 20-Feb-08 20-Feb-08 20-Feb-08 26-Jan-08 26-Jan-08 26-Jan-08 26-Jan-08 26-Jan-08 26-Jan-08 26-Jan-08 26-Jan-08 25-Jan-08 25-Jan-08 25-Jan-08 25-Jan-08 25-Jan-08 25-Jan-08 21-Jan-08 21-Jan-08 21-Jan-08 21-Jan-08 21-Jan-08 21-Jan-08 21-Jan-08 21-Jan-08 21-Jan-08 21-Jan-08 21-Jan-08

Smicridea (S.) decora Smicridea (S.) decora Smicridea (S.) cf. annulicornis Smicridea (S.) cf. manzanara Smicridea (S.) cf. manzanara Smicridea (S.) cf. manzanara Smicridea (S.) cf. manzanara Smicridea (S.) cf. manzanara Smicridea (S.) cf. manzanara Smicridea (S.) cf. manzanara Smicridea (S.) cf. manzanara Smicridea (S.) cf. manzanara Smicridea (S.) cf. manzanara Smicridea (S.) cf. manzanara Smicridea (S.) frequens Smicridea (S.) frequens Smicridea (S.) frequens Smicridea (S.) frequens Smicridea (S.) frequens Smicridea (S.) cf. annulicornis Smicridea (S.) cf. annulicornis Smicridea (S.) cf. annulicornis Smicridea (S.) cf. annulicornis Smicridea (S.) cf. decora Smicridea (S.) cf. decora Smicridea (S.) cf. decora Smicridea (S.) cf. decora Smicridea (S.) frequens Smicridea (S.) smilodon Smicridea (S.) cf. annulicornis Smicridea (S.) cf. annulicornis Smicridea (S.) cf. annulicornis Smicridea (S.) decora Smicridea (S.) cf. annulicornis Smicridea (S.) cf. annulicornis Smicridea (S.) cf. annulicornis Smicridea (S.) cf. manzanara Smicridea (S.) frequens Smicridea (S.) frequens Smicridea (S.) frequens Smicridea (S.) frequens Smicridea (S.) cf. annulicornis Smicridea (S.) cf. decora Smicridea (S.) cf. decora Smicridea (S.) frequens Smicridea (S.) frequens Smicridea (S.) frequens Smicridea (S.) cf. annulicornis Smicridea (S.) cf. annulicornis Smicridea (S.) cf. annulicornis Smicridea (S.) cf. annulicornis Smicridea (S.) cf. annulicornis Smicridea (S.) cf. manzanara Smicridea (S.) cf. manzanara Smicridea (S.) cf. manzanara Smicridea (S.) cf. manzanara Smicridea (S.) cf. manzanara Smicridea (S.) cf. manzanara

CL, Rio El Volcan CL, El Volcan Valley, Quebrada Salto del Agua, 7km W Villa del Valle CL, RN Rio Los Cipreses, Quebrada, El Ranchillo CL, RN Rio Los Cipreses, Quebrada, El Ranchillo CL, RN Rio Los Cipreses, Quebrada, El Ranchillo CL, RN Rio Los Cipreses, Quebrada, El Ranchillo CL, RN Rio Los Cipreses, Quebrada, El Ranchillo CL, RN Rio Los Cipreses, Quebrada, El Ranchillo CL, RN Rio Los Cipreses, Quebrada, El Ranchillo CL, RN Rio Los Cipreses, Quebrada, El Ranchillo CL, RN Rio Los Cipreses, Quebrada, El Ranchillo CL, RN Rio Los Cipreses, Quebrada, El Ranchillo CL, RN Rio Los Cipreses, Quebrada, El Ranchillo CL, RN Rio Los Cipreses, Quebrada, El Ranchillo CL, RN Rio Los Cipreses, Quebrada, El Ranchillo CL, RN Rio Los Cipreses, Quebrada, El Ranchillo CL, RN Rio Los Cipreses, Quebrada, El Ranchillo CL, RN Rio Los Cipreses, Quebrada, El Ranchillo CL, RN Rio Los Cipreses, Quebrada, El Ranchillo CL, Rio Claro at Ruta 5 CL, Rio Claro at Ruta 5 CL, Rio Claro at Ruta 5 CL, Rio Claro at Ruta 5 CL, Rio Claro at Ruta 5 CL, Rio Claro at Ruta 5 CL, Rio Claro at Ruta 5 CL, Rio Claro at Ruta 5 CL, Rio Claro at Ruta 5 CL, RN Radal Siete Tazas, Mirador Siete Tazas CL, RN Radal Siete Tazas, Rio Claro, Bridge at Parque Ingles CL, RN Radal Siete Tazas, Rio Claro, Bridge at Parque Ingles CL, RN Radal Siete Tazas, Rio Claro, Bridge at Parque Ingles CL, RN Radal Siete Tazas, Rio Claro, Bridge at Parque Ingles CL, RN Los Ruiles, Rio Curanilahue, 20km SE of Chanco CL, RN Los Ruiles, Rio Curanilahue, 20km SE of Chanco CL, RN Los Ruiles, Rio Curanilahue, 20km SE of Chanco CL, RN Los Ruiles, Rio Curanilahue, 20km SE of Chanco CL, RN Los Ruiles, Rio Curanilahue, 20km SE of Chanco CL, RN Los Ruiles, Rio Curanilahue, 20km SE of Chanco CL, RN Los Ruiles, Rio Curanilahue, 20km SE of Chanco CL, RN Los Ruiles, Rio Curanilahue, 20km SE of Chanco CL, Rio Ancoa, about 25km E of Linares CL, Rio Ancoa, about 25km E of Linares CL, Rio Ancoa, about 25km E of Linares CL, Rio Ancoa, about 25km E of Linares CL, Rio Ancoa, about 25km E of Linares CL, Rio Ancoa, about 25km E of Linares CL, PN La Campana, Estero Rabuco at El Casino CL, PN La Campana, Estero Rabuco at El Casino CL, PN La Campana, Estero Rabuco at El Casino CL, PN La Campana, Estero Rabuco at El Casino CL, PN La Campana, Estero Rabuco at El Casino CL, PN La Campana, Estero Rabuco at El Casino CL, PN La Campana, Estero Rabuco at El Casino CL, PN La Campana, Estero Rabuco at El Casino CL, PN La Campana, Estero Rabuco at El Casino CL, PN La Campana, Estero Rabuco at El Casino CL, PN La Campana, Estero Rabuco at El Casino

Pauls et al_JNABS_Barcoding Chilean Smicridea_Appendix 1.xls

UMSP SpecimenID / BOLD sample ID UMSP000113593 UMSP000113366 UMSP000113436 UMSP000113435 UMSP000210950 UMSP000113368 UMSP000113369 UMSP000113374 UMSP000113492 UMSP000113493 UMSP000113494 UMSP000113495 UMSP000113496 UMSP000113497 UMSP000113438 UMSP000130213 UMSP000130214 UMSP000130215 UMSP000130216 UMSP000131062 UMSP000131063 UMSP000131064 UMSP000132525 UMSP000131204 UMSP000131205 UMSP000131208 UMSP000131209 UMSP000131210 UMSP000127150 UMSP000131211 UMSP000131213 UMSP000131214 UMSP000131215 UMSP000130455 UMSP000130456 UMSP000130457 UMSP000113554 UMSP000113457 UMSP000132890 UMSP000113456 UMSP000113458 UMSP000130743 UMSP000130814 UMSP000130817 UMSP000130838 UMSP000130839 UMSP000130840 UMSP000130420 UMSP000130422 UMSP000130429 UMSP000113488 UMSP000113489 UMSP000113420 UMSP000130406 UMSP000130408 UMSP000130417 UMSP000130418 UMSP000130419

Collector*

Stage

Haplotype

RWH, PKM, SUP RWH, PKM, SUP RWH, PKM, SUP RWH, PKM, SUP RWH, PKM, SUP RWH, PKM, SUP RWH, PKM, SUP RWH, PKM, SUP RWH, PKM, SUP RWH, PKM, SUP RWH, PKM, SUP RWH, PKM, SUP RWH, PKM, SUP RWH, PKM, SUP RWH, PKM, SUP RWH, PKM, SUP RWH, PKM, SUP RWH, PKM, SUP RWH, PKM, SUP SUP, PKM, MLCL SUP, PKM, MLCL SUP, PKM, MLCL SUP, PKM, MLCL SUP, PKM, MLCL SUP, PKM, MLCL SUP, PKM, MLCL SUP, PKM, MLCL SUP, PKM, MLCL SUP, PKM, MLCL SUP, PKM, MLCL SUP, PKM, MLCL SUP, PKM, MLCL SUP, PKM, MLCL RWH, PKM, SUP RWH, PKM, SUP RWH, PKM, SUP RWH, PKM, SUP RWH, PKM, SUP RWH, PKM, SUP RWH, PKM, SUP RWH, PKM, SUP RWH, PKM, SUP RWH, PKM, SUP RWH, PKM, SUP RWH, PKM, SUP RWH, PKM, SUP RWH, PKM, SUP RWH, PKM, SUP RWH, PKM, SUP RWH, PKM, SUP RWH, PKM, SUP RWH, PKM, SUP RWH, PKM, SUP RWH, PKM, SUP RWH, PKM, SUP RWH, PKM, SUP RWH, PKM, SUP RWH, PKM, SUP

Male Female Female Male Male Female Female Female Larva Larva Larva Larva Larva Larva Male Male Male Male Male Male Male Male Male Male Male Female Female Male Male Male Male Male Female Male Male Male Male Male Male Female Female Male Male Male Male Male Male Male Male Male Larva Larva Male Male Male Male Male Male

n142 Hap016 Hap003 Hap028 n119 Hap026 Hap027 Hap027 Hap026 Hap026 Hap026 Hap026 Hap026 Hap026 Hap022 n101 n101 n101 n101 n125 Hap093 n126 Hap093 n132 Hap094 n133 Hap094 n104 n120 n126 n125 Hap001 n142 Hap001 Hap001 n124 n136 n101 n101 n108 Hap023 Hap090 n134 n135 n103 Hap021 Hap025 n121 n122 n123 Hap001 Hap002 Hap029 Hap030 Hap030 Hap030 Hap031 Hap030

GenBank Sequence Accession Origin HM065224 MN HM065236 B HM065150 B HM065200 B HM065201 B HM065197 B HM065198 B HM065199 B HM065191 B HM065192 B HM065193 B HM065194 B HM065195 B HM065196 B HM065258 B HM065240 MN HM065241 MN HM065242 MN HM065243 MN HM065103 MN HM065104 MN HM065105 MN HM065118 MN HM065183 B HM065184 B HM065181 MN HM065182 MN HM065247 MN HM065479 B HM065106 MN HM065107 MN HM065108 MN HM065226 MN HM065099 MN HM065100 MN HM065101 MN HM065190 B HM065256 B HM065252 MN HM065255 B HM065257 B HM065102 MN HM065185 B HM065186 B HM065244 MN HM065245 MN HM065246 MN HM065096 MN HM065097 MN HM065098 MN HM065175 B HM065176 B HM065207 B HM065209 B HM065187 MN HM065210 B HM065188 MN HM065189 MN

Page 1

Appendix 1: Chilean Smicridea (S. ) specimens used in the study. Region Site

Elevation

Latitude

Longitude

Date

Taxon

AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN

490 m asl 490 m asl 490 m asl 490 m asl 490 m asl 490 m asl 670 m asl 670 m asl 670 m asl 670 m asl 670 m asl 670 m asl 670 m asl 670 m asl 670 m asl 670 m asl 670 m asl 670 m asl 964 m asl 964 m asl 964 m asl 964 m asl 964 m asl 964 m asl 1000 m asl 1000 m asl 1000 m asl 1000 m asl 1000 m asl 1000 m asl 1000 m asl 930 m asl 930 m asl 930 m asl 930 m asl 930 m asl 930 m asl 930 m asl 930 m asl 930 m asl 930 m asl 930 m asl 930 m asl 930 m asl 930 m asl 930 m asl 930 m asl 930 m asl 930 m asl 1000 m asl 1000 m asl 1000 m asl 1000 m asl 1000 m asl 1000 m asl 1000 m asl 1000 m asl 1000 m asl

35°56.583'S 35°56.583'S 35°56.583'S 35°56.583'S 35°56.583'S 35°56.583'S 36°40.189'S 36°40.189'S 36°40.189'S 36°40.189'S 36°40.189'S 36°40.189'S 36°40.189'S 36°40.189'S 36°40.189'S 36°40.189'S 36°40.189'S 36°40.189'S 36°53.355'S 36°53.355'S 36°53.355'S 36°53.355'S 36°53.355'S 36°53.355'S 36°54'32"S 36°54'32"S 36°54'32"S 36°54'32"S 36°54'32"S 36°54'32"S 36°54'32"S 37°23.780'S 37°23.780'S 37°23.780'S 37°23.780'S 37°23.780'S 37°23.780'S 37°23.780'S 37°23'47"S 37°23'47"S 37°23'47"S 37°23'47"S 37°23'47"S 37°23'47"S 37°23'47"S 37°23'47"S 37°23'47"S 37°23'47"S 37°23'47"S 37°23'53"S 37°23'53"S 37°23'53"S 37°23'53"S 37°23'53"S 37°23'53"S 37°23'53"S 37°23'53"S 37°23'53"S

72°05.061'W 72°05.061'W 72°05.061'W 72°05.061'W 72°05.061'W 72°05.061'W 71°18.372'W 71°18.372'W 71°18.372'W 71°18.372'W 71°18.372'W 71°18.372'W 71°18.372'W 71°18.372'W 71°18.372'W 71°18.372'W 71°18.372'W 71°18.372'W 71°33.756'W 71°33.756'W 71°33.756'W 71°33.756'W 71°33.756'W 71°33.756'W 071°31'37"W 071°31'37"W 071°31'37"W 071°31'37"W 071°31'37"W 071°31'37"W 071°31'37"W 71°27.262'W 71°27.262'W 71°27.262'W 71°27.262'W 71°27.262'W 71°27.262'W 71°27.262'W 071°27'16"W 071°27'16"W 071°27'16"W 071°27'16"W 071°27'16"W 071°27'16"W 071°27'16"W 071°27'16"W 071°27'16"W 071°27'16"W 071°27'16"W 071°27'13"W 071°27'13"W 071°27'13"W 071°27'13"W 071°27'13"W 071°27'13"W 071°27'13"W 071°27'13"W 071°27'13"W

21-Jan-08 21-Jan-08 21-Jan-08 21-Jan-08 21-Jan-08 21-Jan-08 18-Feb-08 18-Feb-08 18-Feb-08 18-Feb-08 18-Feb-08 18-Feb-08 18-Feb-08 18-Feb-08 18-Feb-08 18-Feb-08 18-Feb-08 18-Feb-08 17-Feb-08 17-Feb-08 17-Feb-08 17-Feb-08 17-Feb-08 17-Feb-08 15-Feb-05 15-Feb-05 15-Feb-05 15-Feb-05 15-Feb-05 15-Feb-05 15-Feb-05 13-Feb-05 13-Feb-05 13-Feb-05 13-Feb-05 13-Feb-05 13-Feb-05 13-Feb-05 13-Feb-05 13-Feb-05 13-Feb-05 13-Feb-05 13-Feb-05 13-Feb-05 13-Feb-05 13-Feb-05 13-Feb-05 13-Feb-05 13-Feb-05 14-Feb-05 14-Feb-05 14-Feb-05 14-Feb-05 14-Feb-05 14-Feb-05 14-Feb-05 14-Feb-05 14-Feb-05

Smicridea (S.) cf. manzanara Smicridea (S.) cf. manzanara Smicridea (S.) cf. manzanara Smicridea (S.) cf. manzanara Smicridea (S.) cf. manzanara Smicridea (S.) cf. manzanara Smicridea (S.) cf. annulicornis Smicridea (S.) cf. annulicornis Smicridea (S.) cf. annulicornis Smicridea (S.) cf. annulicornis Smicridea (S.) decora Smicridea (S.) decora Smicridea (S.) decora Smicridea (S.) decora Smicridea (S.) decora Smicridea (S.) decora Smicridea (S.) decora Smicridea (S.) frequens Smicridea (S.) annulicornis Smicridea (S.) cf. annulicornis Smicridea (S.) cf. annulicornis Smicridea (S.) cf. annulicornis Smicridea (S.) frequens Smicridea (S.) frequens Smicridea (S.) cf. annulicornis Smicridea (S.) cf. annulicornis Smicridea (S.) cf. annulicornis Smicridea (S.) cf. annulicornis Smicridea (S.) cf. annulicornis Smicridea (S.) cf. annulicornis Smicridea (S.) cf. annulicornis Smicridea (S.) anticura Smicridea (S.) anticura Smicridea (S.) anticura Smicridea (S.) anticura Smicridea (S.) decora Smicridea (S.) decora Smicridea (S.) decora Smicridea (S.) anticura Smicridea (S.) pucara Smicridea (S.) pucara Smicridea (S.) pucara Smicridea (S.) pucara Smicridea (S.) pucara Smicridea (S.) pucara Smicridea (S.) pucara Smicridea (S.) pucara Smicridea (S.) pucara Smicridea (S.) pucara Smicridea (S.) pucara Smicridea (S.) pucara Smicridea (S.) pucara Smicridea (S.) pucara Smicridea (S.) pucara Smicridea (S.) pucara Smicridea (S.) pucara Smicridea (S.) pucara Smicridea (S.) pucara

CL, PN La Campana, Estero Rabuco at El Casino CL, PN La Campana, Estero Rabuco at El Casino CL, PN La Campana, Estero Rabuco at El Casino CL, PN La Campana, Estero Rabuco at El Casino CL, PN La Campana, Estero Rabuco at El Casino CL, PN La Campana, Estero Rabuco at El Casino CL, Rio Nuble, La Puntilla CL, Rio Nuble, La Puntilla CL, Rio Nuble, La Puntilla CL, Rio Nuble, La Puntilla CL, Rio Nuble, La Puntilla CL, Rio Nuble, La Puntilla CL, Rio Nuble, La Puntilla CL, Rio Nuble, La Puntilla CL, Rio Nuble, La Puntilla CL, Rio Nuble, La Puntilla CL, Rio Nuble, La Puntilla CL, Rio Nuble, La Puntilla CL, Rio Renegado, 32 km E of Recinto CL, Rio Renegado, 32 km E of Recinto CL, Rio Renegado, 32 km E of Recinto CL, Rio Renegado, 32 km E of Recinto CL, Rio Renegado, 32 km E of Recinto CL, Rio Renegado, 32 km E of Recinto CL, Río Renegado, 14.5 km E Recinto CL, Río Renegado, 14.5 km E Recinto CL, Río Renegado, 14.5 km E Recinto CL, Río Renegado, 14.5 km E Recinto CL, Río Renegado, 14.5 km E Recinto CL, Río Renegado, 14.5 km E Recinto CL, Río Renegado, 14.5 km E Recinto CL, Salto Trubunleo, 4.5km E of El Albanico CL, Salto Trubunleo, 4.5km E of El Albanico CL, Salto Trubunleo, 4.5km E of El Albanico CL, Salto Trubunleo, 4.5km E of El Albanico CL, Salto Trubunleo, 4.5km E of El Albanico CL, Salto Trubunleo, 4.5km E of El Albanico CL, Salto Trubunleo, 4.5km E of El Albanico CL, Salto Trubunleo, 4.5km E of El Albanico CL, Salto Trubunleo, 4.5km E of El Albanico CL, Salto Trubunleo, 4.5km E of El Albanico CL, Salto Trubunleo, 4.5km E of El Albanico CL, Salto Trubunleo, 4.5km E of El Albanico CL, Salto Trubunleo, 4.5km E of El Albanico CL, Salto Trubunleo, 4.5km E of El Albanico CL, Salto Trubunleo, 4.5km E of El Albanico CL, Salto Trubunleo, 4.5km E of El Albanico CL, Salto Trubunleo, 4.5km E of El Albanico CL, Salto Trubunleo, 4.5km E of El Albanico CL, spring creeks & tribs. to Río Trubunleo, 5 km E El Abanico CL, spring creeks & tribs. to Río Trubunleo, 5 km E El Abanico CL, spring creeks & tribs. to Río Trubunleo, 5 km E El Abanico CL, spring creeks & tribs. to Río Trubunleo, 5 km E El Abanico CL, spring creeks & tribs. to Río Trubunleo, 5 km E El Abanico CL, spring creeks & tribs. to Río Trubunleo, 5 km E El Abanico CL, spring creeks & tribs. to Río Trubunleo, 5 km E El Abanico CL, spring creeks & tribs. to Río Trubunleo, 5 km E El Abanico CL, spring creeks & tribs. to Río Trubunleo, 5 km E El Abanico

Pauls et al_JNABS_Barcoding Chilean Smicridea_Appendix 1.xls

UMSP SpecimenID / BOLD sample ID UMSP000113423 UMSP000113485 UMSP000113486 UMSP000113487 UMSP000113490 UMSP000113491 UMSP000132660 UMSP000132661 UMSP000132662 UMSP000113483 UMSP000113594 UMSP000132596 UMSP000132597 UMSP000132598 UMSP000132599 UMSP000113482 UMSP000113484 UMSP000132765 UMSP000133009 UMSP000133010 UMSP000133011 UMSP000133012 UMSP000133019 UMSP000133021 UMSP000106672 UMSP000106673 UMSP000106674 UMSP000106675 UMSP000106676 UMSP000106677 UMSP000106679 UMSP000083872 UMSP000083873 UMSP000083874 UMSP000083875 UMSP000117739 UMSP000117740 UMSP000117741 UMSP000083876 UMSP000117721 UMSP000117722 UMSP000117723 UMSP000117724 UMSP000117725 UMSP000117726 UMSP000117727 UMSP000117728 UMSP000117729 UMSP000117730 UMSP000037580 UMSP000037581 UMSP000037582 UMSP000117696 UMSP000117697 UMSP000117698 UMSP000117699 UMSP000117700 UMSP000117701

Collector*

Stage

Haplotype

RWH, PKM, SUP RWH, PKM, SUP RWH, PKM, SUP RWH, PKM, SUP RWH, PKM, SUP RWH, PKM, SUP MLCL, PKM, SUP MLCL, PKM, SUP MLCL, PKM, SUP MLCL, PKM, SUP MLCL, PKM, SUP MLCL, PKM, SUP MLCL, PKM, SUP MLCL, PKM, SUP MLCL, PKM, SUP MLCL, PKM, SUP MLCL, PKM, SUP MLCL, PKM, SUP SUP, PKM, MLCL SUP, PKM, MLCL SUP, PKM, MLCL SUP, PKM, MLCL SUP, PKM, MLCL SUP, PKM, MLCL RWH, RJB, MLCL RWH, RJB, MLCL RWH, RJB, MLCL RWH, RJB, MLCL RWH, RJB, MLCL RWH, RJB, MLCL RWH, RJB, MLCL RWH, RJB, MLCL RWH, RJB, MLCL RWH, RJB, MLCL RWH, RJB, MLCL RWH, RJB, MLCL RWH, RJB, MLCL RWH, RJB, MLCL RWH, RJB, MLCL RWH, RJB, MLCL RWH, RJB, MLCL RWH, RJB, MLCL RWH, RJB, MLCL RWH, RJB, MLCL RWH, RJB, MLCL RWH, RJB, MLCL RWH, RJB, MLCL RWH, RJB, MLCL RWH, RJB, MLCL RWH, RJB, MLCL RWH, RJB, MLCL RWH, RJB, MLCL RWH, RJB, MLCL RWH, RJB, MLCL RWH, RJB, MLCL RWH, RJB, MLCL RWH, RJB, MLCL RWH, RJB, MLCL

Larva Larva Larva Larva Larva Larva Male Male Male Larva Male Male Male Male Male Larva Larva Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Female Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male

Hap029 Hap030 Hap030 Hap031 Hap030 Hap030 n128 Hap001 Hap093 Hap001 Hap015 Hap015 Hap015 Hap015 Hap015 Hap015 Hap015 n105 Hap086 Hap007 Hap001 Hap005 n109 Hap022 Hap007 Hap086 Hap001 Hap007 Hap007 Hap007 n131 Hap014 Hap014 Hap014 Hap014 n143 n144 Hap017 Hap014 Hap073 Hap074 Hap075 Hap075 Hap075 Hap075 Hap075 Hap075 Hap075 Hap075 Hap075 Hap075 Hap075 Hap075 Hap075 Hap075 Hap075 Hap075 Hap075

GenBank Sequence Accession Origin HM065208 B HM065202 B HM065203 B HM065204 B HM065205 B HM065206 B HM065119 MN HM065120 MN HM065121 MN HM065142 B HM065225 MN HM065227 MN HM065228 MN HM065229 MN HM065230 MN HM065231 B HM065232 B HM065251 MN HM065062 MN HM065124 MN HM065125 MN HM065126 MN HM065253 MN HM065254 MN HM065143 B HM065144 B HM065145 B HM065146 B HM065147 B HM065148 B HM065149 B HM065083 B HM065084 B HM065085 B HM065086 B HM065233 B HM065234 B HM065235 B HM065082 B HM065447 B HM065448 B HM065449 B HM065450 B HM065451 B HM065452 B HM065453 B HM065454 B HM065455 B HM065456 B HM065460 B HM065461 B HM065462 B HM065463 B HM065464 B HM065465 B HM065466 B HM065467 B HM065468 B

Page 2

Appendix 1: Chilean Smicridea (S. ) specimens used in the study. Region Site

Elevation

Latitude

Longitude

Date

Taxon

AN AN AN AN AN AN AN AN AN AN AN CR CR CR CR CR CR CR CR CR CR CR CR CR CR CR CR CR CR CR CR CR CR CR CR CR CR CR CR CR CR CR CR CR CR CR CR CR CR CR CR CR CR CR CR CR CR CR

1000 m asl 525 m asl 525 m asl 525 m asl 450 m asl 450 m asl 450 m asl 450 m asl 450 m asl 450 m asl 450 m asl 1070 m asl 1070 m asl 1070 m asl 1070 m asl 1070 m asl 1070 m asl 1070 m asl 1070 m asl 1070 m asl 1070 m asl 1070 m asl 1070 m asl 1070 m asl 1070 m asl 1070 m asl 1070 m asl 1070 m asl 1070 m asl 1070 m asl 1070 m asl 190 m asl 190 m asl 1070 m asl 1070 m asl 1070 m asl 1070 m asl 1070 m asl 1070 m asl 1070 m asl 1070 m asl 1070 m asl 1070 m asl 1070 m asl 1070 m asl 1070 m asl 1070 m asl 1070 m asl 1070 m asl 1070 m asl 1070 m asl 1070 m asl 1100 m asl 1100 m asl 1100 m asl 1100 m asl 1100 m asl 1100 m asl

37°23'53"S 37°37.678'S 37°37.678'S 37°37.678'S 37°38'42"S 37°38'42"S 37°38'42"S 37°38'42"S 37°38'42"S 37°38'42"S 37°38'42"S 37°47.859'S 37°47.859'S 37°47.859'S 37°47.859'S 37°47.859'S 37°47.859'S 37°47.859'S 37°47.859'S 37°47.859'S 37°47.859'S 37°47.859'S 37°47.859'S 37°47.859'S 37°47.859'S 37°47.859'S 37°47.859'S 37°47.859'S 37°47.859'S 37°47.859'S 37°47.859'S 37°47'30"S 37°47'30"S 37°47'52"S 37°47'52"S 37°47'52"S 37°47'52"S 37°47'52"S 37°47'52"S 37°47'52"S 37°47'52"S 37°47'52"S 37°47'52"S 37°47'52"S 37°47'52"S 37°47'52"S 37°47'52"S 37°47'52"S 37°47'52"S 37°47'52"S 37°47'52"S 37°47'52"S 37°48.500'S 37°48.500'S 37°48.500'S 37°48.500'S 37°48.500'S 37°48.500'S

071°27'13"W 71°39.015'W 71°39.015'W 71°39.015'W 071°41'46"W 071°41'46"W 071°41'46"W 071°41'46"W 071°41'46"W 071°41'46"W 071°41'46"W 73°02.617'W 73°02.617'W 73°02.617'W 73°02.617'W 73°02.617'W 73°02.617'W 73°02.617'W 73°02.617'W 73°02.617'W 73°02.617'W 73°02.617'W 73°02.617'W 73°02.617'W 73°02.617'W 73°02.617'W 73°02.617'W 73°02.617'W 73°02.617'W 73°02.617'W 73°02.617'W 73°21'42"W 73°21'42"W 073°02'37"W 073°02'37"W 073°02'37"W 073°02'37"W 073°02'37"W 073°02'37"W 073°02'37"W 073°02'37"W 073°02'37"W 073°02'37"W 073°02'37"W 073°02'37"W 073°02'37"W 073°02'37"W 073°02'37"W 073°02'37"W 073°02'37"W 073°02'37"W 073°02'37"W 73°00.662'W 73°00.662'W 73°00.662'W 73°00.662'W 73°00.662'W 73°00.662'W

14-Feb-05 27-Jan-08 27-Jan-08 27-Jan-08 15-Jan-00 15-Jan-00 15-Jan-00 15-Jan-00 15-Jan-00 15-Jan-00 15-Jan-00 29-Jan-08 29-Jan-08 29-Jan-08 29-Jan-08 29-Jan-08 29-Jan-08 29-Jan-08 29-Jan-08 29-Jan-08 29-Jan-08 29-Jan-08 29-Jan-08 12-Feb-05 12-Feb-05 12-Feb-05 12-Feb-05 12-Feb-05 12-Feb-05 12-Feb-05 29-Jan-08 31-Jan-08 31-Jan-08 11-Feb-05 11-Feb-05 11-Feb-05 11-Feb-05 11-Feb-05 11-Feb-05 11-Feb-05 11-Feb-05 11-Feb-05 11-Feb-05 11-Feb-05 11-Feb-05 11-Feb-05 11-Feb-05 11-Feb-05 11-Feb-05 11-Feb-05 11-Feb-05 11-Feb-05 30-Jan-08 30-Jan-08 30-Jan-08 30-Jan-08 30-Jan-08 30-Jan-08

Smicridea (S.) pucara Smicridea (S.) cf. annulicornis Smicridea (S.) cf. annulicornis Smicridea (S.) cf. anticura Smicridea (S.) cf. annulicornis Smicridea (S.) cf. annulicornis Smicridea (S.) cf. annulicornis Smicridea (S.) cf. annulicornis Smicridea (S.) cf. annulicornis Smicridea (S.) cf. annulicornis Smicridea (S.) cf. annulicornis Smicridea (S.) manzanara Smicridea (S.) manzanara Smicridea (S.) manzanara Smicridea (S.) manzanara Smicridea (S.) manzanara Smicridea (S.) manzanara Smicridea (S.) manzanara Smicridea (S.) mucronata Smicridea (S.) mucronata Smicridea (S.) pucara Smicridea (S.) pucara Smicridea (S.) redunca Smicridea (S.) turgida Smicridea (S.) turgida Smicridea (S.) turgida Smicridea (S.) turgida Smicridea (S.) turgida Smicridea (S.) turgida Smicridea (S.) turgida Smicridea (S.) turgida Smicridea (S.) annulicornis Smicridea (S.) annulicornis Smicridea (S.) manzanara Smicridea (S.) mucronata Smicridea (S.) mucronata Smicridea (S.) mucronata Smicridea (S.) mucronata Smicridea (S.) mucronata Smicridea (S.) mucronata Smicridea (S.) mucronata Smicridea (S.) mucronata Smicridea (S.) pucara Smicridea (S.) pucara Smicridea (S.) pucara Smicridea (S.) pucara Smicridea (S.) pucara Smicridea (S.) pucara Smicridea (S.) pucara Smicridea (S.) pucara Smicridea (S.) turgida Smicridea (S.) turgida Smicridea (S.) mucronata Smicridea (S.) mucronata Smicridea (S.) mucronata Smicridea (S.) mucronata Smicridea (S.) mucronata Smicridea (S.) mucronata

CL, spring creeks & tribs. to Río Trubunleo, 5 km E El Abanico CL, Rio Huequecura at Camping Santa Laura CL, Rio Huequecura at Camping Santa Laura CL, Rio Huequecura at Camping Santa Laura CL, Río Huequecura in El Guachi CL, Río Huequecura in El Guachi CL, Río Huequecura in El Guachi CL, Río Huequecura in El Guachi CL, Río Huequecura in El Guachi CL, Río Huequecura in El Guachi CL, Río Huequecura in El Guachi CL, 2km W of PN Nahuelbuta Entrance, Estero Pichinahuel CL, 2km W of PN Nahuelbuta Entrance, Estero Pichinahuel CL, 2km W of PN Nahuelbuta Entrance, Estero Pichinahuel CL, 2km W of PN Nahuelbuta Entrance, Estero Pichinahuel CL, 2km W of PN Nahuelbuta Entrance, Estero Pichinahuel CL, 2km W of PN Nahuelbuta Entrance, Estero Pichinahuel CL, 2km W of PN Nahuelbuta Entrance, Estero Pichinahuel CL, 2km W of PN Nahuelbuta Entrance, Estero Pichinahuel CL, 2km W of PN Nahuelbuta Entrance, Estero Pichinahuel CL, 2km W of PN Nahuelbuta Entrance, Estero Pichinahuel CL, 2km W of PN Nahuelbuta Entrance, Estero Pichinahuel CL, 2km W of PN Nahuelbuta Entrance, Estero Pichinahuel CL, 2km W of PN Nahuelbuta Entrance, Estero Pichinahuel CL, 2km W of PN Nahuelbuta Entrance, Estero Pichinahuel CL, 2km W of PN Nahuelbuta Entrance, Estero Pichinahuel CL, 2km W of PN Nahuelbuta Entrance, Estero Pichinahuel CL, 2km W of PN Nahuelbuta Entrance, Estero Pichinahuel CL, 2km W of PN Nahuelbuta Entrance, Estero Pichinahuel CL, 2km W of PN Nahuelbuta Entrance, Estero Pichinahuel CL, 2km W of PN Nahuelbuta Entrance, Estero Pichinahuel CL, Rio Cayucupil, Puente Esperanza CL, Rio Cayucupil, Puente Esperanza CL, 2km W of PN Nahuelbuta Entrance, Estero Pichinahuel CL, 2km W of PN Nahuelbuta Entrance, Estero Pichinahuel CL, 2km W of PN Nahuelbuta Entrance, Estero Pichinahuel CL, 2km W of PN Nahuelbuta Entrance, Estero Pichinahuel CL, 2km W of PN Nahuelbuta Entrance, Estero Pichinahuel CL, 2km W of PN Nahuelbuta Entrance, Estero Pichinahuel CL, 2km W of PN Nahuelbuta Entrance, Estero Pichinahuel CL, 2km W of PN Nahuelbuta Entrance, Estero Pichinahuel CL, 2km W of PN Nahuelbuta Entrance, Estero Pichinahuel CL, 2km W of PN Nahuelbuta Entrance, Estero Pichinahuel CL, 2km W of PN Nahuelbuta Entrance, Estero Pichinahuel CL, 2km W of PN Nahuelbuta Entrance, Estero Pichinahuel CL, 2km W of PN Nahuelbuta Entrance, Estero Pichinahuel CL, 2km W of PN Nahuelbuta Entrance, Estero Pichinahuel CL, 2km W of PN Nahuelbuta Entrance, Estero Pichinahuel CL, 2km W of PN Nahuelbuta Entrance, Estero Pichinahuel CL, 2km W of PN Nahuelbuta Entrance, Estero Pichinahuel CL, 2km W of PN Nahuelbuta Entrance, Estero Pichinahuel CL, 2km W of PN Nahuelbuta Entrance, Estero Pichinahuel CL, PN Nahuelbuta, Estero Los Gringos CL, PN Nahuelbuta, Estero Los Gringos CL, PN Nahuelbuta, Estero Los Gringos CL, PN Nahuelbuta, Estero Los Gringos CL, PN Nahuelbuta, Estero Los Gringos CL, PN Nahuelbuta, Estero Los Gringos

Pauls et al_JNABS_Barcoding Chilean Smicridea_Appendix 1.xls

UMSP SpecimenID / BOLD sample ID UMSP000117702 UMSP000132826 UMSP000132827 UMSP000124933 UMSP000038275 UMSP000038276 UMSP000038277 UMSP000038278 UMSP000038279 UMSP000038280 UMSP000038281 UMSP000131548 UMSP000113498 UMSP000113499 UMSP000113500 UMSP000113501 UMSP000113502 UMSP000113503 UMSP000131406 UMSP000131407 UMSP000113270 UMSP000113504 UMSP000131544 UMSP000117784 UMSP000117785 UMSP000117786 UMSP000117787 UMSP000117788 UMSP000117789 UMSP000125005 UMSP000131546 UMSP000113515 UMSP000113516 UMSP000117783 UMSP000085458 UMSP000085459 UMSP000085460 UMSP000085461 UMSP000085462 UMSP000085463 UMSP000085464 UMSP000085465 UMSP000117757 UMSP000117758 UMSP000117759 UMSP000117761 UMSP000117763 UMSP000117764 UMSP000117765 UMSP000117766 UMSP000117799 UMSP000117802 UMSP000113462 UMSP000113463 UMSP000113464 UMSP000113465 UMSP000113466 UMSP000113467

Collector*

Stage

Haplotype

RWH, RJB, MLCL RWH, PKM, SUP RWH, PKM, SUP RWH, PKM, SUP RWH , FJMQ RWH , FJMQ RWH , FJMQ RWH , FJMQ RWH , FJMQ RWH , FJMQ RWH , FJMQ RWH, PKM, SUP RWH, PKM, SUP RWH, PKM, SUP RWH, PKM, SUP RWH, PKM, SUP RWH, PKM, SUP RWH, PKM, SUP RWH, PKM, SUP RWH, PKM, SUP RWH, PKM, SUP RWH, PKM, SUP RWH, PKM, SUP RWH, RJB, MLCL RWH, RJB, MLCL RWH, RJB, MLCL RWH, RJB, MLCL RWH, RJB, MLCL RWH, RJB, MLCL RWH, RJB, MLCL RWH, PKM, SUP RWH, PKM, SUP RWH, PKM, SUP RWH, RJB, MLCL RWH, RJB, MLCL RWH, RJB, MLCL RWH, RJB, MLCL RWH, RJB, MLCL RWH, RJB, MLCL RWH, RJB, MLCL RWH, RJB, MLCL RWH, RJB, MLCL RWH, RJB, MLCL RWH, RJB, MLCL RWH, RJB, MLCL RWH, RJB, MLCL RWH, RJB, MLCL RWH, RJB, MLCL RWH, RJB, MLCL RWH, RJB, MLCL RWH, RJB, MLCL RWH, RJB, MLCL RWH, PKM, SUP RWH, PKM, SUP RWH, PKM, SUP RWH, PKM, SUP RWH, PKM, SUP RWH, PKM, SUP

Male Male Male Male Male Male Male Male Male Male Male Male Larva Larva Larva Larva Larva Larva Male Male Male Larva Male Male Male Male Male Male Male Male Female Larva Larva Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male

Hap075 Hap007 Hap007 Hap037 Hap090 Hap090 Hap090 Hap090 Hap090 Hap090 Hap090 Hap032 Hap032 Hap032 Hap032 Hap032 Hap032 Hap032 Hap040 n100 Hap069 Hap069 Hap081 Hap096 Hap096 Hap096 Hap096 Hap096 n110 Hap096 Hap096 Hap012 Hap012 Hap032 Hap039 Hap040 Hap040 Hap040 Hap040 Hap040 Hap041 Hap040 Hap069 Hap070 Hap071 Hap071 Hap069 Hap072 Hap071 Hap069 Hap096 Hap096 Hap040 Hap042 Hap042 Hap043 Hap044 Hap042

GenBank Sequence Accession Origin HM065469 B HM065122 MN HM065123 MN HM065178 B HM065135 B HM065136 B HM065137 B HM065138 B HM065139 B HM065140 B HM065141 B HM065275 MN HM065277 B HM065278 B HM065279 B HM065280 B HM065281 B HM065282 B HM065295 MN HM065296 MN HM065420 MN HM065427 B HM065476 B HM065487 B HM065488 B HM065489 B HM065490 B HM065485 B HM065491 B HM065494 B HM065486 MN HM065063 B HM065064 B HM065276 B HM065364 B HM065365 B HM065366 B HM065367 B HM065368 B HM065369 B HM065370 B HM065371 B HM065472 B HM065473 B HM065421 B HM065422 B HM065423 B HM065424 B HM065425 B HM065426 B HM065492 B HM065493 B HM065311 B HM065312 B HM065313 B HM065314 B HM065315 B HM065316 B

Page 3

Appendix 1: Chilean Smicridea (S. ) specimens used in the study. Region Site

Elevation

Latitude

Longitude

Date

Taxon

CR CR AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN CR CR CR CR CR CR AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN

1100 m asl 1100 m asl 515 m asl 515 m asl 515 m asl 515 m asl 515 m asl 515 m asl 515 m asl 515 m asl 515 m asl 515 m asl 515 m asl 515 m asl 515 m asl 515 m asl 515 m asl 515 m asl 515 m asl 1390 m asl 1390 m asl 1390 m asl 1390 m asl 1390 m asl 1390 m asl 517 m asl 517 m asl 517 m asl 517 m asl 517 m asl 517 m asl 517 m asl 517 m asl 517 m asl 517 m asl 517 m asl 900 m asl 900 m asl 900 m asl 900 m asl 910 m asl 910 m asl 910 m asl 910 m asl 910 m asl 910 m asl 910 m asl 910 m asl 900 m asl 900 m asl 900 m asl 900 m asl 900 m asl 900 m asl 900 m asl 900 m asl 900 m asl 900 m asl

37°48.500'S 37°48.500'S 37°49.958'S 37°49.958'S 37°49.958'S 37°49.958'S 37°49.958'S 37°49.958'S 37°49.958'S 37°49.958'S 37°49.958'S 37°49.958'S 37°49.958'S 37°49.958'S 37°49.958'S 37°49.958'S 37°49.958'S 37°49.958'S 37°49.958'S 37°49'47"S 37°49'47"S 37°49'47"S 37°49'47"S 37°49'47"S 37°49'47"S 37°51.655'S 37°51.655'S 37°51.655'S 37°51.655'S 37°51.655'S 37°51.655'S 37°51.655'S 37°51.655'S 37°51.655'S 37°51.655'S 37°51.655'S 37°52.731'S 37°52.731'S 37°52.731'S 37°52.731'S 37°52.830'S 37°52.830'S 37°52.830'S 37°52.830'S 37°52.830'S 37°52.830'S 37°52.830'S 37°52.830'S 37°52'44"S 37°52'44"S 37°52'44"S 37°52'44"S 37°52'44"S 37°52'44"S 37°52'44"S 37°52'44"S 37°52'44"S 37°52'44"S

73°00.662'W 73°00.662'W 71°34.308'W 71°34.308'W 71°34.308'W 71°34.308'W 71°34.308'W 71°34.308'W 71°34.308'W 71°34.308'W 71°34.308'W 71°34.308'W 71°34.308'W 71°34.308'W 71°34.308'W 71°34.308'W 71°34.308'W 71°34.308'W 71°34.308'W 73°00'32"W 73°00'32"W 73°00'32"W 73°00'32"W 73°00'32"W 73°00'32"W 71°36.279'W 71°36.279'W 71°36.279'W 71°36.279'W 71°36.279'W 71°36.279'W 71°36.279'W 71°36.279'W 71°36.279'W 71°36.279'W 71°36.279'W 71°30.785'W 71°30.785'W 71°30.785'W 71°30.785'W 71°30.594'W 71°30.594'W 71°30.594'W 71°30.594'W 71°30.594'W 71°30.594'W 71°30.594'W 71°30.594'W 071°30'47"W 071°30'47"W 071°30'47"W 071°30'47"W 071°30'47"W 071°30'47"W 071°30'47"W 071°30'47"W 071°30'47"W 071°30'47"W

30-Jan-08 30-Jan-08 28-Jan-08 28-Jan-08 28-Jan-08 28-Jan-08 28-Jan-08 28-Jan-08 28-Jan-08 28-Jan-08 28-Jan-08 28-Jan-08 28-Jan-08 28-Jan-08 28-Jan-08 28-Jan-08 28-Jan-08 28-Jan-08 28-Jan-08 30-Jan-08 30-Jan-08 30-Jan-08 30-Jan-08 30-Jan-08 30-Jan-08 16-Jan-00 16-Jan-00 16-Jan-00 16-Jan-00 16-Jan-00 28-Jan-08 28-Jan-08 28-Jan-08 28-Jan-08 16-Jan-00 28-Jan-08 25-Jan-05 25-Jan-05 25-Jan-05 25-Jan-05 26-Jan-05 26-Jan-05 26-Jan-05 26-Jan-05 26-Jan-05 26-Jan-05 26-Jan-05 26-Jan-05 25-Jan-05 25-Jan-05 25-Jan-05 25-Jan-05 25-Jan-05 25-Jan-05 25-Jan-05 25-Jan-05 25-Jan-05 25-Jan-05

Smicridea (S.) pucara Smicridea (S.) pucara Smicridea (S.) cf. annulicornis Smicridea (S.) cf. annulicornis Smicridea (S.) cf. annulicornis Smicridea (S.) cf. annulicornis Smicridea (S.) cf. anticura Smicridea (S.) frequens Smicridea (S.) frequens Smicridea (S.) frequens Smicridea (S.) mucronata Smicridea (S.) mucronata Smicridea (S.) mucronata Smicridea (S.) mucronata Smicridea (S.) mucronata Smicridea (S.) mucronata Smicridea (S.) pucara Smicridea (S.) pucara Smicridea (S.) smilodon Smicridea (S.) pucara Smicridea (S.) pucara Smicridea (S.) pucara Smicridea (S.) pucara Smicridea (S.) pucara Smicridea (S.) pucara Smicridea (S.) figueroai Smicridea (S.) frequens Smicridea (S.) mucronata Smicridea (S.) mucronata Smicridea (S.) mucronata Smicridea (S.) mucronata Smicridea (S.) pucara Smicridea (S.) pucara Smicridea (S.) pucara Smicridea (S.) smilodon Smicridea (S.) smilodon Smicridea (S.) mucronata Smicridea (S.) mucronata Smicridea (S.) mucronata Smicridea (S.) mucronata Smicridea (S.) mucronata Smicridea (S.) mucronata Smicridea (S.) mucronata Smicridea (S.) mucronata Smicridea (S.) mucronata Smicridea (S.) mucronata Smicridea (S.) mucronata Smicridea (S.) mucronata Smicridea (S.) mucronata Smicridea (S.) mucronata Smicridea (S.) mucronata Smicridea (S.) mucronata Smicridea (S.) mucronata Smicridea (S.) mucronata Smicridea (S.) mucronata Smicridea (S.) mucronata Smicridea (S.) mucronata Smicridea (S.) mucronata

CL, PN Nahuelbuta, Estero Los Gringos CL, PN Nahuelbuta, Estero Los Gringos CL, Tributary to Rio Queuco at Puente Pitril, 9.8 km NE of Ralco CL, Tributary to Rio Queuco at Puente Pitril, 9.8 km NE of Ralco CL, Tributary to Rio Queuco at Puente Pitril, 9.8 km NE of Ralco CL, Tributary to Rio Queuco at Puente Pitril, 9.8 km NE of Ralco CL, Tributary to Rio Queuco at Puente Pitril, 9.8 km NE of Ralco CL, Tributary to Rio Queuco at Puente Pitril, 9.8 km NE of Ralco CL, Tributary to Rio Queuco at Puente Pitril, 9.8 km NE of Ralco CL, Tributary to Rio Queuco at Puente Pitril, 9.8 km NE of Ralco CL, Tributary to Rio Queuco at Puente Pitril, 9.8 km NE of Ralco CL, Tributary to Rio Queuco at Puente Pitril, 9.8 km NE of Ralco CL, Tributary to Rio Queuco at Puente Pitril, 9.8 km NE of Ralco CL, Tributary to Rio Queuco at Puente Pitril, 9.8 km NE of Ralco CL, Tributary to Rio Queuco at Puente Pitril, 9.8 km NE of Ralco CL, Tributary to Rio Queuco at Puente Pitril, 9.8 km NE of Ralco CL, Tributary to Rio Queuco at Puente Pitril, 9.8 km NE of Ralco CL, Tributary to Rio Queuco at Puente Pitril, 9.8 km NE of Ralco CL, Tributary to Rio Queuco at Puente Pitril, 9.8 km NE of Ralco CL, PN Nahuelbuta, Estero Pehuenco, pantrap CL, PN Nahuelbuta, Estero Pehuenco, pantrap CL, PN Nahuelbuta, Estero Pehuenco, pantrap CL, PN Nahuelbuta, Estero Pehuenco, pantrap CL, PN Nahuelbuta, Estero Pehuenco, pantrap CL, PN Nahuelbuta, Estero Pehuenco, pantrap CL, small trib to Rio Queuco, 4.6km E Ralco CL, small trib to Rio Queuco, 4.6km E Ralco CL, small trib to Rio Queuco, 4.6km E Ralco CL, small trib to Rio Queuco, 4.6km E Ralco CL, small trib to Rio Queuco, 4.6km E Ralco CL, small trib to Rio Queuco, 4.6km E Ralco CL, small trib to Rio Queuco, 4.6km E Ralco CL, small trib to Rio Queuco, 4.6km E Ralco CL, small trib to Rio Queuco, 4.6km E Ralco CL, small trib to Rio Queuco, 4.6km E Ralco CL, small trib to Rio Queuco, 4.6km E Ralco CL, Rio Pangue, 11km E on rd to Ralco CL, Rio Pangue, 11km E on rd to Ralco CL, Rio Pangue, 11km E on rd to Ralco CL, Rio Pangue, 11km E on rd to Ralco CL, Estero Colluco, 11km E rd to Ralco CL, Estero Colluco, 11km E rd to Ralco CL, Estero Colluco, 11km E rd to Ralco CL, Estero Colluco, 11km E rd to Ralco CL, Estero Colluco, 11km E rd to Ralco CL, Estero Colluco, 11km E rd to Ralco CL, Estero Colluco, 11km E rd to Ralco CL, Estero Colluco, 11km E rd to Ralco CL, Rio Pangue, 11km E on rd to Ralco CL, Rio Pangue, 11km E on rd to Ralco CL, Rio Pangue, 11km E on rd to Ralco CL, Rio Pangue, 11km E on rd to Ralco CL, Rio Pangue, 11km E on rd to Ralco CL, Rio Pangue, 11km E on rd to Ralco CL, Rio Pangue, 11km E on rd to Ralco CL, Rio Pangue, 11km E on rd to Ralco CL, Rio Pangue, 11km E on rd to Ralco CL, Rio Pangue, 11km E on rd to Ralco

Pauls et al_JNABS_Barcoding Chilean Smicridea_Appendix 1.xls

UMSP SpecimenID / BOLD sample ID UMSP000113563 UMSP000127170 UMSP000131319 UMSP000131321 UMSP000113478 UMSP000113479 UMSP000113473 UMSP000113477 UMSP000113470 UMSP000113469 UMSP000113468 UMSP000113471 UMSP000113472 UMSP000113474 UMSP000113475 UMSP000113476 UMSP000113480 UMSP000113481 UMSP000124983 UMSP000113570 UMSP000127163 UMSP000127164 UMSP000127165 UMSP000127166 UMSP000127167 UMSP000037748 UMSP000037756 UMSP000037749 UMSP000037754 UMSP000037755 UMSP000113416 UMSP000113512 UMSP000113513 UMSP000113514 UMSP000037775 UMSP000113511 UMSP000117915 UMSP000117916 UMSP000117917 UMSP000117918 UMSP000118010 UMSP000118011 UMSP000118015 UMSP000118017 UMSP000118018 UMSP000118020 UMSP000118021 UMSP000118073 UMSP000117905 UMSP000117906 UMSP000117907 UMSP000117908 UMSP000117909 UMSP000117910 UMSP000117911 UMSP000117912 UMSP000117913 UMSP000117914

Collector*

Stage

Haplotype

RWH, PKM, SUP RWH, PKM, SUP RWH, PKM, SUP RWH, PKM, SUP RWH, PKM, SUP RWH, PKM, SUP RWH, PKM, SUP RWH, PKM, SUP RWH, PKM, SUP RWH, PKM, SUP RWH, PKM, SUP RWH, PKM, SUP RWH, PKM, SUP RWH, PKM, SUP RWH, PKM, SUP RWH, PKM, SUP RWH, PKM, SUP RWH, PKM, SUP RWH, PKM, SUP RWH, PKM, SUP RWH, PKM, SUP RWH, PKM, SUP RWH, PKM, SUP RWH, PKM, SUP RWH, PKM, SUP RWH , FJMQ RWH , FJMQ RWH , FJMQ RWH , FJMQ RWH , FJMQ RWH, PKM, SUP RWH, PKM, SUP RWH, PKM, SUP RWH, PKM, SUP RWH , FJMQ RWH, PKM, SUP RWH, RJB, MLCL RWH, RJB, MLCL RWH, RJB, MLCL RWH, RJB, MLCL RWH, RJB, MLCL RWH, RJB, MLCL RWH, RJB, MLCL RWH, RJB, MLCL RWH, RJB, MLCL RWH, RJB, MLCL RWH, RJB, MLCL RWH, RJB, MLCL RWH, RJB, MLCL RWH, RJB, MLCL RWH, RJB, MLCL RWH, RJB, MLCL RWH, RJB, MLCL RWH, RJB, MLCL RWH, RJB, MLCL RWH, RJB, MLCL RWH, RJB, MLCL RWH, RJB, MLCL

Male Male Male Male Female Female Female Male Female ? Male Male Male Male ? ? Female Female Female Male Male Male Male Male Male Male Male Male Male Male Male Larva Larva Larva Male Larva Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male

Hap077 Hap077 n127 Hap005 Hap010 Hap007 Hap037 Hap022 Hap024 n107 Hap035 Hap036 Hap036 Hap036 Hap038 Hap036 Hap065 Hap065 Hap083 n145 n146 n117 Hap077 Hap079 n118 Hap018 Hap022 Hap036 Hap033 Hap036 Hap036 Hap065 Hap065 Hap065 Hap083 Hap082 Hap033 Hap033 Hap033 Hap033 Hap033 Hap033 Hap033 Hap033 Hap033 Hap033 Hap033 Hap033 Hap033 Hap033 Hap033 Hap034 Hap033 Hap033 Hap033 Hap033 Hap034 Hap033

GenBank Sequence Accession Origin HM065435 B HM065436 B HM065109 MN HM065110 MN HM065159 B HM065160 B HM065179 B HM065262 B HM065261 B HM065260 B HM065350 B HM065351 B HM065352 B HM065353 B HM065354 B HM065355 B HM065470 B HM065471 B HM065482 B HM065437 B HM065438 B HM065439 B HM065440 B HM065441 B HM065442 B HM065239 B HM065259 B HM065347 B HM065348 B HM065349 B HM065286 MN HM065457 B HM065458 B HM065459 B HM065480 B HM065481 B HM065334 B HM065335 B HM065336 B HM065337 B HM065372 B HM065373 B HM065374 B HM065375 B HM065376 B HM065377 B HM065378 B HM065285 B HM065324 B HM065325 B HM065326 B HM065327 B HM065328 B HM065329 B HM065330 B HM065331 B HM065332 B HM065333 B

Page 4

Appendix 1: Chilean Smicridea (S. ) specimens used in the study. Region Site

Elevation

Latitude

Longitude

Date

Taxon

CR CR CR AN AN AN AN AN AN AN AN AN AN AN AN AN VC VC VC VC AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN

7 m asl 7 m asl 7 m asl 830 m asl 830 m asl 830 m asl 830 m asl 830 m asl 830 m asl 830 m asl 830 m asl 830 m asl 830 m asl 830 m asl 830 m asl 830 m asl 325 m asl 325 m asl 325 m asl 325 m asl 300 m asl 300 m asl 300 m asl 300 m asl 300 m asl 300 m asl 300 m asl 300 m asl 300 m asl 300 m asl 770 m asl 770 m asl 770 m asl 770 m asl 770 m asl 770 m asl 475 m asl 475 m asl 475 m asl 475 m asl 475 m asl 475 m asl 475 m asl 475 m asl 290 m asl 290 m asl 290 m asl 290 m asl 290 m asl 290 m asl 290 m asl 290 m asl 290 m asl 290 m asl 290 m asl 290 m asl 290 m asl 290 m asl

37°56.823'S 37°56.823'S 37°56.823'S 38°28.218'S 38°28.218'S 38°28.218'S 38°28.218'S 38°28.218'S 38°28.218'S 38°28.218'S 38°28.218'S 38°28.218'S 38°28.218'S 38°28.218'S 38°28.218'S 38°28.218'S 38°29.669'S 38°29.669'S 38°29.669'S 38°29'40"S 39°03.790'S 39°03.790'S 39°03.790'S 39°03.790'S 39°03.790'S 39°03.790'S 39°03.790'S 39°03.790'S 39°03.790'S 39°03.790'S 39°09.447'S 39°09.447'S 39°09.447'S 39°09.447'S 39°09.447'S 39°09.447'S 39°12'36"S 39°12'36"S 39°12'36"S 39°12'36"S 39°12'36"S 39°12'36"S 39°12'36"S 39°12'36"S 39°14.657'S 39°14.657'S 39°14.657'S 39°14.657'S 39°14.657'S 39°14.657'S 39°14.657'S 39°14.657'S 39°14.657'S 39°14.657'S 39°14.657'S 39°14.657'S 39°14.657'S 39°14.657'S

73°15.429'W 73°15.429'W 73°15.429'W 71°38.744'W 71°38.744'W 71°38.744'W 71°38.744'W 71°38.744'W 71°38.744'W 71°38.744'W 71°38.744'W 71°38.744'W 71°38.744'W 71°38.744'W 71°38.744'W 71°38.744'W 72°15.300'W 72°15.300'W 72°15.300'W 072°15'18"W 71°58.620'W 71°58.620'W 71°58.620'W 71°58.620'W 71°58.620'W 71°58.620'W 71°58.620'W 71°58.620'W 71°58.620'W 71°58.620'W 71°43.084'W 71°43.084'W 71°43.084'W 71°43.084'W 71°43.084'W 71°43.084'W 071°43'59"W 071°43'59"W 071°43'59"W 071°43'59"W 071°43'59"W 071°43'59"W 071°43'59"W 071°43'59"W 71°50.622'W 71°50.622'W 71°50.622'W 71°50.622'W 71°50.622'W 71°50.622'W 71°50.622'W 71°50.622'W 71°50.622'W 71°50.622'W 71°50.622'W 71°50.622'W 71°50.622'W 71°50.622'W

31-Jan-08 31-Jan-08 31-Jan-08 1-Feb-08 1-Feb-08 1-Feb-08 1-Feb-08 1-Feb-08 1-Feb-08 1-Feb-08 1-Feb-08 1-Feb-08 1-Feb-08 1-Feb-08 1-Feb-08 1-Feb-08 28-Jan-05 28-Jan-05 28-Jan-05 28-Jan-05 9-Feb-05 9-Feb-05 22-Jan-00 22-Jan-00 9-Feb-05 22-Jan-00 22-Jan-00 9-Feb-05 9-Feb-05 9-Feb-05 3-Feb-08 3-Feb-08 3-Feb-08 3-Feb-08 3-Feb-08 3-Feb-08 23-Jan-00 23-Jan-00 23-Jan-00 23-Jan-00 23-Jan-00 23-Jan-00 23-Jan-00 23-Jan-00 2-Feb-08 8-Feb-05 2-Feb-08 8-Feb-05 8-Feb-05 8-Feb-05 8-Feb-05 8-Feb-05 8-Feb-05 8-Feb-05 8-Feb-05 8-Feb-05 8-Feb-05 8-Feb-05

Smicridea (S.) cf. annulicornis Smicridea (S.) cf. annulicornis Smicridea (S.) cf. annulicornis Smicridea (S.) cf. annulicornis Smicridea (S.) cf. annulicornis Smicridea (S.) mucronata Smicridea (S.) mucronata Smicridea (S.) mucronata Smicridea (S.) mucronata Smicridea (S.) mucronata Smicridea (S.) mucronata Smicridea (S.) mucronata Smicridea (S.) mucronata Smicridea (S.) mucronata Smicridea (S.) mucronata Smicridea (S.) mucronata Smicridea (S.) cf. annulicornis Smicridea (S.) cf. annulicornis Smicridea (S.) penai Smicridea (S.) cf. penai Smicridea (S.) cf. anticura Smicridea (S.) decora Smicridea (S.) frequens Smicridea (S.) frequens Smicridea (S.) frequens Smicridea (S.) mucronata Smicridea (S.) mucronata Smicridea (S.) mucronata Smicridea (S.) mucronata Smicridea (S.) pucara Smicridea (S.) annulicornis Smicridea (S.) annulicornis Smicridea (S.) annulicornis Smicridea (S.) cf. annulicornis Smicridea (S.) mucronata Smicridea (S.) pucara Smicridea (S.) cf. annulicornis Smicridea (S.) cf. annulicornis Smicridea (S.) cf. annulicornis Smicridea (S.) cf. annulicornis Smicridea (S.) cf. annulicornis Smicridea (S.) cf. annulicornis Smicridea (S.) cf. annulicornis Smicridea (S.) cf. annulicornis Smicridea (S.) annulicornis Smicridea (S.) annulicornis Smicridea (S.) cf. annulicornis Smicridea (S.) cf. annulicornis Smicridea (S.) cf. annulicornis Smicridea (S.) cf. annulicornis Smicridea (S.) cf. annulicornis Smicridea (S.) cf. annulicornis Smicridea (S.) cf. annulicornis Smicridea (S.) cf. annulicornis Smicridea (S.) cf. annulicornis Smicridea (S.) cf. annulicornis Smicridea (S.) cf. annulicornis Smicridea (S.) cf. annulicornis

CL, Lago Lanalhue, North shore about 7 km NW of Contulmo CL, Lago Lanalhue, North shore about 7 km NW of Contulmo CL, Lago Lanalhue, North shore about 7 km NW of Contulmo CL, Rio Colorado, HWY81 West of Malalcahuello CL, Rio Colorado, HWY81 West of Malalcahuello CL, Rio Colorado, HWY81 West of Malalcahuello CL, Rio Colorado, HWY81 West of Malalcahuello CL, Rio Colorado, HWY81 West of Malalcahuello CL, Rio Colorado, HWY81 West of Malalcahuello CL, Rio Colorado, HWY81 West of Malalcahuello CL, Rio Colorado, HWY81 West of Malalcahuello CL, Rio Colorado, HWY81 West of Malalcahuello CL, Rio Colorado, HWY81 West of Malalcahuello CL, Rio Colorado, HWY81 West of Malalcahuello CL, Rio Colorado, HWY81 West of Malalcahuello CL, Rio Colorado, HWY81 West of Malalcahuello CL, Estero Peu-Peu, E of Lautero CL, Estero Peu-Peu, E of Lautero CL, Estero Peu-Peu, E of Lautero CL, Estero Peu-Peu, E of Lautero CL, Lago Colico, waterfall near shore CL, Lago Colico, waterfall near shore CL, Lago Colico, waterfall near shore CL, Lago Colico, waterfall near shore CL, Lago Colico, waterfall near shore CL, Lago Colico, waterfall near shore CL, Lago Colico, waterfall near shore CL, Lago Colico, waterfall near shore CL, Lago Colico, waterfall near shore CL, Lago Colico, waterfall near shore CL, 1km W of PN Huerquehue, Refugio Tinquilco, Rio Huerquehue CL, 1km W of PN Huerquehue, Refugio Tinquilco, Rio Huerquehue CL, 1km W of PN Huerquehue, Refugio Tinquilco, Rio Huerquehue CL, 1km W of PN Huerquehue, Refugio Tinquilco, Rio Huerquehue CL, 1km W of PN Huerquehue, Refugio Tinquilco, Rio Huerquehue CL, 1km W of PN Huerquehue, Refugio Tinquilco, Rio Huerquehue CL, Tres Saltos de Huife Río Quinchón CL, Tres Saltos de Huife Río Quinchón CL, Tres Saltos de Huife Río Quinchón CL, Tres Saltos de Huife Río Quinchón CL, Tres Saltos de Huife Río Quinchón CL, Tres Saltos de Huife Río Quinchón CL, Tres Saltos de Huife Río Quinchón CL, Tres Saltos de Huife Río Quinchón CL, Camping "La Puntilla", Confl. Rio Caburga & Rio Carhuello CL, Camping "La Puntilla", Confl. Rio Caburga & Rio Carhuello CL, Camping "La Puntilla", Confl. Rio Caburga & Rio Carhuello CL, Camping "La Puntilla", Confl. Rio Caburga & Rio Carhuello CL, Camping "La Puntilla", Confl. Rio Caburga & Rio Carhuello CL, Camping "La Puntilla", Confl. Rio Caburga & Rio Carhuello CL, Camping "La Puntilla", Confl. Rio Caburga & Rio Carhuello CL, Camping "La Puntilla", Confl. Rio Caburga & Rio Carhuello CL, Camping "La Puntilla", Confl. Rio Caburga & Rio Carhuello CL, Camping "La Puntilla", Confl. Rio Caburga & Rio Carhuello CL, Camping "La Puntilla", Confl. Rio Caburga & Rio Carhuello CL, Camping "La Puntilla", Confl. Rio Caburga & Rio Carhuello CL, Camping "La Puntilla", Confl. Rio Caburga & Rio Carhuello CL, Camping "La Puntilla", Confl. Rio Caburga & Rio Carhuello

Pauls et al_JNABS_Barcoding Chilean Smicridea_Appendix 1.xls

UMSP SpecimenID / BOLD sample ID UMSP000131813 UMSP000131814 UMSP000131815 UMSP000131737 UMSP000131776 UMSP000113573 UMSP000113574 UMSP000113575 UMSP000113576 UMSP000113577 UMSP000113578 UMSP000113579 UMSP000113580 UMSP000131587 UMSP000131588 UMSP000131589 UMSP000085506 UMSP000085507 UMSP000085504 UMSP000085505 UMSP000105626 UMSP000038199 UMSP000037773 UMSP000037774 UMSP000105629 UMSP000037771 UMSP000037772 UMSP000105627 UMSP000105628 UMSP000037590 UMSP000113461 UMSP000113459 UMSP000113460 UMSP000132146 UMSP000132147 UMSP000113569 UMSP000037979 UMSP000037980 UMSP000037981 UMSP000037982 UMSP000037983 UMSP000037984 UMSP000037985 UMSP000037986 UMSP000113424 UMSP000113517 UMSP000113425 UMSP000132009 UMSP000210607 UMSP000210608 UMSP000210609 UMSP000210610 UMSP000210611 UMSP000210612 UMSP000210613 UMSP000210614 UMSP000210615 UMSP000210659m

Collector*

Stage

Haplotype

RWH, PKM, SUP RWH, PKM, SUP RWH, PKM, SUP RWH, PKM, SUP RWH, PKM, SUP RWH, PKM, SUP RWH, PKM, SUP RWH, PKM, SUP RWH, PKM, SUP RWH, PKM, SUP RWH, PKM, SUP RWH, PKM, SUP RWH, PKM, SUP RWH, PKM, SUP RWH, PKM, SUP RWH, PKM, SUP RWH, RJB, MLCL RWH, RJB, MLCL RWH, RJB, MLCL RWH, RJB, MLCL RWH, RJB, MLCL RWH, RJB, MLCL RWH, RJB, MLCL RWH, RJB, MLCL RWH, RJB, MLCL RWH, RJB, MLCL RWH, RJB, MLCL RWH, RJB, MLCL RWH, RJB, MLCL RWH, RJB, MLCL RWH, PKM, SUP RWH, PKM, SUP RWH, PKM, SUP RWH, PKM, SUP RWH, PKM, SUP RWH, PKM, SUP RWH , FJMQ RWH , FJMQ RWH , FJMQ RWH , FJMQ RWH , FJMQ RWH , FJMQ RWH , FJMQ RWH , FJMQ RWH, PKM, SUP RWH, RJB, MLCL RWH, PKM, SUP RWH, RJB, MLCL RWH, RJB, MLCL RWH, RJB, MLCL RWH, RJB, MLCL RWH, RJB, MLCL RWH, RJB, MLCL RWH, RJB, MLCL RWH, RJB, MLCL RWH, RJB, MLCL RWH, RJB, MLCL RWH, RJB, MLCL

Male Male Male Male Female Male Male Male Male Male Male Male Male Male Male Male Female Female Male Male Female Male Female Female Female Male Male Male Male Male Male Female Female Male Female Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male

n130 Hap005 Hap007 n129 Hap006 n099 Hap033 Hap033 n099 Hap033 Hap033 n097 Hap033 Hap033 Hap033 Hap033 Hap006 Hap093 n116 Hap058 Hap037 n144 Hap025 Hap022 Hap022 Hap051 Hap033 Hap033 Hap051 Hap080 Hap009 Hap008 Hap009 Hap006 Hap033 Hap076 Hap087 Hap005 Hap088 Hap005 Hap005 n130 Hap005 Hap089 Hap004 n141 Hap005 Hap005 Hap005 Hap006 Hap086 Hap006 Hap091 Hap086 Hap092 Hap006 Hap093 Hap093

GenBank Sequence Accession Origin HM065113 MN HM065114 MN HM065115 MN HM065111 MN HM065112 MN HM065287 MN HM065288 MN HM065289 MN HM065290 MN HM065291 MN HM065292 MN HM065293 MN HM065294 MN HM065297 MN HM065298 MN HM065299 MN HM065127 B HM065095 B HM065398 B HM065212 B HM065180 B HM065237 B HM065265 B HM065266 B HM065267 B HM065379 B HM065380 B HM065381 B HM065382 B HM065475 B HM065070 B HM065068 B HM065069 B HM065117 MN HM065300 MN HM065474 B HM065151 B HM065152 B HM065153 B HM065154 B HM065155 B HM065156 B HM065157 B HM065158 B HM065072 B HM065071 B HM065161 B HM065116 MN HM065162 B HM065163 B HM065164 B HM065165 B HM065166 B HM065167 B HM065168 B HM065169 B HM065170 B HM065172 B

Page 5

Appendix 1: Chilean Smicridea (S. ) specimens used in the study. Region Site

Elevation

Latitude

Longitude

Date

Taxon

AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN CR CR CR CR CR CR CR CR CR CR CR AN AN AN AN AN AN AN AN AN AN AN AN AN AN AN CR CR CR CR

290 m asl 290 m asl 290 m asl 290 m asl 290 m asl 290 m asl 290 m asl 290 m asl 290 m asl 290 m asl 290 m asl 290 m asl 250 m asl 250 m asl 250 m asl 250 m asl 250 m asl 250 m asl 250 m asl 250 m asl 250 m asl 50 m asl 50 m asl 50 m asl 50 m asl 50 m asl 50 m asl 50 m asl 895 m asl 895 m asl 895 m asl 895 m asl 895 m asl 895 m asl 895 m asl 895 m asl 895 m asl 895 m asl 895 m asl 351 m asl 351 m asl 351 m asl 351 m asl 351 m asl 351 m asl 351 m asl 351 m asl 351 m asl 351 m asl 75 m asl 75 m asl 75 m asl 75 m asl 60 m asl 15 m asl 15 m asl 15 m asl 15 m asl

39°14.657'S 39°14.657'S 39°14.657'S 39°14.657'S 39°14.657'S 39°14.657'S 39°14.657'S 39°14.657'S 39°14.657'S 39°14.657'S 39°14.657'S 39°14.657'S 39°32.426'S 39°32.426'S 39°32.426'S 39°32.426'S 39°32.426'S 39°32.426'S 39°32.426'S 39°32.426'S 39°32.426'S 40°07.266'S 40°07.266'S 40°07.266'S 40°07.266'S 40°07.266'S 40°07'16"S 40°07'16"S 40°11.874'S 40°11.874'S 40°11.874'S 40°11.874'S 40°11.874'S 40°11.874'S 40°11.874'S 40°11.874'S 40°11.874'S 40°11.874'S 40°11.874'S 40°40.006'S 40°40.006'S 40°40.006'S 40°40.006'S 40°40.006'S 40°40.006'S 40°40.006'S 40°40.006'S 40°40.006'S 40°40.006'S 40°41.203'S 40°41.203'S 40°41.203'S 40°41'12"S 41°13.006'S 41°16.427'S 41°16.427'S 41°16.427'S 41°16.427'S

71°50.622'W 71°50.622'W 71°50.622'W 71°50.622'W 71°50.622'W 71°50.622'W 71°50.622'W 71°50.622'W 71°50.622'W 71°50.622'W 71°50.622'W 71°50.622'W 72°03.098'W 72°03.098'W 72°03.098'W 72°03.098'W 72°03.098'W 72°03.098'W 72°03.098'W 72°03.098'W 72°03.098'W 72°24.710'W 72°24.710'W 72°24.710'W 72°24.710'W 72°24.710'W 072°24'43"W 072°24'43"W 73°26.217'W 73°26.217'W 73°26.217'W 73°26.217'W 73°26.217'W 73°26.217'W 73°26.217'W 73°26.217'W 73°26.217'W 73°26.217'W 73°26.217'W 72°10.548'W 72°10.548'W 72°10.548'W 72°10.548'W 72°10.548'W 72°10.548'W 72°10.548'W 72°10.548'W 72°10.548'W 72°10.548'W 72°20.028'W 72°20.028'W 72°20.028'W 072°20'02"W 72°33.483'W 73°47.939'W 73°47.939'W 73°47.939'W 73°47.939'W

2-Feb-08 8-Feb-05 2-Feb-08 2-Feb-08 2-Feb-08 2-Feb-08 2-Feb-08 2-Feb-08 2-Feb-08 2-Feb-08 2-Feb-08 2-Feb-08 7-Feb-05 7-Feb-05 7-Feb-05 7-Feb-05 7-Feb-05 7-Feb-05 7-Feb-05 7-Feb-05 7-Feb-05 4-Feb-08 4-Feb-08 4-Feb-08 4-Feb-08 4-Feb-08 6-Feb-05 6-Feb-05 5-Feb-08 5-Feb-08 5-Feb-08 5-Feb-08 5-Feb-08 5-Feb-08 5-Feb-08 5-Feb-08 5-Feb-08 5-Feb-08 5-Feb-08 16-Feb-08 16-Feb-08 16-Feb-08 16-Feb-08 16-Feb-08 16-Feb-08 16-Feb-08 16-Feb-08 16-Feb-08 16-Feb-08 30-Jan-05 30-Jan-05 30-Jan-05 30-Jan-05 6-Feb-08 7-Feb-08 7-Feb-08 7-Feb-08 7-Feb-08

Smicridea (S.) cf. annulicornis Smicridea (S.) cf. annulicornis Smicridea (S.) frequens Smicridea (S.) penai Smicridea (S.) penai Smicridea (S.) penai Smicridea (S.) penai Smicridea (S.) penai Smicridea (S.) penai Smicridea (S.) penai Smicridea (S.) penai Smicridea (S.) penai Smicridea (S.) mucronata Smicridea (S.) mucronata Smicridea (S.) mucronata Smicridea (S.) mucronata Smicridea (S.) mucronata Smicridea (S.) mucronata Smicridea (S.) mucronata Smicridea (S.) mucronata Smicridea (S.) mucronata Smicridea (S.) cf. penai Smicridea (S.) cf. penai Smicridea (S.) cf. penai Smicridea (S.) penai Smicridea (S.) penai Smicridea (S.) penai Smicridea (S.) smilodon Smicridea (S.) lourditae, n. sp. Smicridea (S.) lourditae, n. sp. Smicridea (S.) mucronata Smicridea (S.) patinae , n. sp. Smicridea (S.) pucara Smicridea (S.) pucara Smicridea (S.) pucara Smicridea (S.) pucara Smicridea (S.) pucara Smicridea (S.) pucara Smicridea (S.) pucara Smicridea (S.) annulicornis Smicridea (S.) annulicornis Smicridea (S.) annulicornis Smicridea (S.) annulicornis Smicridea (S.) annulicornis Smicridea (S.) frequens Smicridea (S.) frequens Smicridea (S.) mucronata Smicridea (S.) mucronata Smicridea (S.) mucronata Smicridea (S.) annulicornis Smicridea (S.) annulicornis Smicridea (S.) penai Smicridea (S.) annulicornis Smicridea (S.) cf. annulicornis Smicridea (S.) mucronata Smicridea (S.) pucara Smicridea (S.) pucara Smicridea (S.) pucara

CL, Camping "La Puntilla", Confl. Rio Caburga & Rio Carhuello CL, Camping "La Puntilla", Confl. Rio Caburga & Rio Carhuello CL, Camping "La Puntilla", Confl. Rio Caburga & Rio Carhuello CL, Camping "La Puntilla", Confl. Rio Caburga & Rio Carhuello CL, Camping "La Puntilla", Confl. Rio Caburga & Rio Carhuello CL, Camping "La Puntilla", Confl. Rio Caburga & Rio Carhuello CL, Camping "La Puntilla", Confl. Rio Caburga & Rio Carhuello CL, Camping "La Puntilla", Confl. Rio Caburga & Rio Carhuello CL, Camping "La Puntilla", Confl. Rio Caburga & Rio Carhuello CL, Camping "La Puntilla", Confl. Rio Caburga & Rio Carhuello CL, Camping "La Puntilla", Confl. Rio Caburga & Rio Carhuello CL, Camping "La Puntilla", Confl. Rio Caburga & Rio Carhuello CL, Rio Traitraico, 2.5km SE of Pucura CL, Rio Traitraico, 2.5km SE of Pucura CL, Rio Traitraico, 2.5km SE of Pucura CL, Rio Traitraico, 2.5km SE of Pucura CL, Rio Traitraico, 2.5km SE of Pucura CL, Rio Traitraico, 2.5km SE of Pucura CL, Rio Traitraico, 2.5km SE of Pucura CL, Rio Traitraico, 2.5km SE of Pucura CL, Rio Traitraico, 2.5km SE of Pucura CL, Futrono, Camping Nalcahue CL, Futrono, Camping Nalcahue CL, Futrono, Camping Nalcahue CL, Futrono, Camping Nalcahue CL, Futrono, Camping Nalcahue CL, Futrono, Camping Nalcahue CL, Futrono, Camping Nalcahue CL, MN Alerce Costero, unnamed tributary on trail to Alerce Milenario CL, MN Alerce Costero, unnamed tributary on trail to Alerce Milenario CL, MN Alerce Costero, unnamed tributary on trail to Alerce Milenario CL, MN Alerce Costero, unnamed tributary on trail to Alerce Milenario CL, MN Alerce Costero, unnamed tributary on trail to Alerce Milenario CL, MN Alerce Costero, unnamed tributary on trail to Alerce Milenario CL, MN Alerce Costero, unnamed tributary on trail to Alerce Milenario CL, MN Alerce Costero, unnamed tributary on trail to Alerce Milenario CL, MN Alerce Costero, unnamed tributary on trail to Alerce Milenario CL, MN Alerce Costero, unnamed tributary on trail to Alerce Milenario CL, MN Alerce Costero, unnamed tributary on trail to Alerce Milenario CL, PN Puyehue, Rio Anticura, at Mapuche Campground Playa CL, PN Puyehue, Rio Anticura, at Mapuche Campground Playa CL, PN Puyehue, Rio Anticura, at Mapuche Campground Playa CL, PN Puyehue, Rio Anticura, at Mapuche Campground Playa CL, PN Puyehue, Rio Anticura, at Mapuche Campground Playa CL, PN Puyehue, Rio Anticura, at Mapuche Campground Playa CL, PN Puyehue, Rio Anticura, at Mapuche Campground Playa CL, PN Puyehue, Rio Anticura, at Mapuche Campground Playa CL, PN Puyehue, Rio Anticura, at Mapuche Campground Playa CL, PN Puyehue, Rio Anticura, at Mapuche Campground Playa CL, Río Chanleufú, E end of Lago Puyehue CL, Río Chanleufú, E end of Lago Puyehue CL, Río Chanleufú, E end of Lago Puyehue CL, Río Chanleufú, E end of Lago Puyehue CL, Lago Llanquihue in Ensenada CL, tributary to Rio Llico in Llico Bajo CL, tributary to Rio Llico in Llico Bajo CL, tributary to Rio Llico in Llico Bajo CL, tributary to Rio Llico in Llico Bajo

Pauls et al_JNABS_Barcoding Chilean Smicridea_Appendix 1.xls

UMSP SpecimenID / BOLD sample ID UMSP000113426 UMSP000210659f UMSP000113451 UMSP000124945 UMSP000124960 UMSP000124969 UMSP000127143 UMSP000127144 UMSP000127145 UMSP000127146 UMSP000127147 UMSP000127148 UMSP000105213 UMSP000105214 UMSP000105215 UMSP000105216 UMSP000105217 UMSP000105221 UMSP000105222 UMSP000105223 UMSP000105224 UMSP000106745 UMSP000106747 UMSP000113428 UMSP000106744 UMSP000106746 UMSP000106743 UMSP000106742 UMSP000113377 UMSP000113378 UMSP000113376 UMSP000113375 UMSP000113352 UMSP000113355 UMSP000113358 UMSP000113359 UMSP000113361 UMSP000113363 UMSP000113379 UMSP000132453 UMSP000132454 UMSP000132482 UMSP000132483 UMSP000132502 UMSP000132515 UMSP000132521 UMSP000132514 UMSP000132517 UMSP000132518 UMSP000106944 UMSP000106945 UMSP000106942 UMSP000106943 UMSP000113427 UMSP000113442 UMSP000127168 UMSP000129926 UMSP000127169

Collector*

Stage

Haplotype

RWH, PKM, SUP RWH, RJB, MLCL RWH, PKM, SUP RWH, PKM, SUP RWH, PKM, SUP RWH, PKM, SUP RWH, PKM, SUP RWH, PKM, SUP RWH, PKM, SUP RWH, PKM, SUP RWH, PKM, SUP RWH, PKM, SUP RWH, RJB, MLCL RWH, RJB, MLCL RWH, RJB, MLCL RWH, RJB, MLCL RWH, RJB, MLCL RWH, RJB, MLCL RWH, RJB, MLCL RWH, RJB, MLCL RWH, RJB, MLCL RWH, PKM, SUP RWH, PKM, SUP RWH, PKM, SUP RWH, PKM, SUP RWH, PKM, SUP RWH, RJB, MLCL RWH, RJB, MLCL RWH, PKM, SUP RWH, PKM, SUP RWH, PKM, SUP RWH, PKM, SUP RWH, PKM, SUP RWH, PKM, SUP RWH, PKM, SUP RWH, PKM, SUP RWH, PKM, SUP RWH, PKM, SUP RWH, PKM, SUP SUP, PKM, MLCL SUP, PKM, MLCL SUP, PKM, MLCL SUP, PKM, MLCL SUP, PKM, MLCL SUP, PKM, MLCL SUP, PKM, MLCL SUP, PKM, MLCL SUP, PKM, MLCL SUP, PKM, MLCL RWH, RJB, MLCL RWH, RJB, MLCL RWH, RJB, MLCL RWH, RJB, MLCL RWH, PKM, SUP RWH, PKM, SUP RWH, PKM, SUP RWH, PKM, SUP RWH, PKM, SUP

Female Female Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Female Female Female Female Female Male Male Female Female Female Male Female Male Male Male Female Female Male Male Female Male Male Male Female

Hap006 Hap006 Hap021 n112 n115 n114 n113 n114 n114 n115 n114 n115 Hap052 Hap033 Hap033 Hap033 Hap033 Hap033 Hap033 Hap033 Hap033 Hap058 Hap058 Hap058 Hap057 Hap059 Hap057 Hap083 Hap063 Hap064 Hap045 Hap095 Hap066 Hap067 Hap067 Hap067 Hap067 Hap068 Hap067 Hap011 n137 Hap011 Hap009 Hap011 Hap020 Hap020 Hap051 n098 n098 n139 n141 Hap057 n141 Hap007 Hap049 Hap078 Hap078 Hap078

GenBank Sequence Accession Origin HM065173 B HM065171 B HM065264 B HM065406 B HM065407 B HM065408 B HM065409 B HM065410 B HM065411 B HM065412 B HM065413 B HM065414 B HM065338 B HM065339 B HM065340 B HM065341 B HM065342 B HM065343 B HM065344 B HM065345 B HM065346 B HM065219 B HM065220 B HM065221 B HM065416 B HM065417 B HM065415 B HM065478 B HM065273 B HM065274 B HM065310 B HM065396 B HM065428 B HM065429 B HM065430 B HM065431 B HM065432 B HM065433 B HM065434 B HM065057 MN HM065058 MN HM065059 MN HM065060 MN HM065061 MN HM065249 MN HM065250 MN HM065307 MN HM065308 MN HM065309 MN HM065080 B HM065081 B HM065418 B HM065079 B HM065174 B HM065317 B HM065443 B HM065445 B HM065444 B

Page 6

Appendix 1: Chilean Smicridea (S. ) specimens used in the study. Region Site

Elevation

Latitude

Longitude

Date

Taxon

CR CL, tributary to Rio Llico in Llico Bajo 15 m asl 41°16.427'S 73°47.939'W 7-Feb-08 Smicridea (S.) pucara AN CL, Rio la Poza, 4km SE Lago Llanquihue 160 m asl 41°17.391'S 72°45.488'W 1-Feb-05 Smicridea (S.) cf. penai AN CL, Rio la Poza, 4km SE Lago Llanquihue 160 m asl 41°17.391'S 72°45.488'W 1-Feb-05 Smicridea (S.) cf. penai AN CL, Rio la Poza, 4km SE Lago Llanquihue 160 m asl 41°17.391'S 72°45.488'W 1-Feb-05 Smicridea (S.) cf. penai AN CL, Rio la Poza, 4km SE Lago Llanquihue 160 m asl 41°17.391'S 72°45.488'W 1-Feb-05 Smicridea (S.) cf. penai AN CL, Rio la Poza, 4km SE Lago Llanquihue 160 m asl 41°17.391'S 72°45.488'W 1-Feb-05 Smicridea (S.) cf. penai AN CL, Rio la Poza, 4km SE Lago Llanquihue 160 m asl 41°17.391'S 72°45.488'W 12-Feb-08 Smicridea (S.) cf. penai AN CL, Rio la Poza, 4km SE Lago Llanquihue 160 m asl 41°17.391'S 72°45.488'W 12-Feb-08 Smicridea (S.) mucronata AN CL, Rio la Poza, 4km SE Lago Llanquihue 160 m asl 41°17.391'S 72°45.488'W 12-Feb-08 Smicridea (S.) mucronata AN CL, Rio la Poza, 4km SE Lago Llanquihue 160 m asl 41°17.391'S 72°45.488'W 12-Feb-08 Smicridea (S.) mucronata AN CL, Rio la Poza, 4km SE Lago Llanquihue 160 m asl 41°17.391'S 72°45.488'W 12-Feb-08 Smicridea (S.) mucronata AN CL, Rio la Poza, 4km SE Lago Llanquihue 160 m asl 41°17.391'S 72°45.488'W 12-Feb-08 Smicridea (S.) penai AN CL, Rio la Poza, 4km SE Lago Llanquihue 160 m asl 41°17'23"S 072°45'29"W 1-Feb-05 Smicridea (S.) annulicornis AN CL, Rio la Poza, 4km SE Lago Llanquihue 160 m asl 41°17'23"S 072°45'29"W 1-Feb-05 Smicridea (S.) mucronata AN CL, Rio la Poza, 4km SE Lago Llanquihue 160 m asl 41°17'23"S 072°45'29"W 1-Feb-05 Smicridea (S.) mucronata AN CL, Rio la Poza, 4km SE Lago Llanquihue 160 m asl 41°17'23"S 072°45'29"W 1-Feb-05 Smicridea (S.) mucronata AN CL, Rio la Poza, 4km SE Lago Llanquihue 160 m asl 41°17'23"S 072°45'29"W 1-Feb-05 Smicridea (S.) mucronata AN CL, Rio la Poza, 4km SE Lago Llanquihue 160 m asl 41°17'23"S 072°45'29"W 1-Feb-05 Smicridea (S.) mucronata AN CL, Rio la Poza, 4km SE Lago Llanquihue 160 m asl 41°17'23"S 072°45'29"W 1-Feb-05 Smicridea (S.) mucronata AN CL, Río del Este, ca. 3 km S Ralun 10 m asl 41°23'01"S 072°16'23"W 5-Feb-05 Smicridea (S.) cf. annulicornis AN CL, Río del Este, ca. 3 km S Ralun 10 m asl 41°23'01"S 072°16'23"W 5-Feb-05 Smicridea (S.) cf. annulicornis AN CL, Río del Este, ca. 3 km S Ralun 10 m asl 41°23'01"S 072°16'23"W 5-Feb-05 Smicridea (S.) cf. annulicornis AN CL, Río del Este, ca. 3 km S Ralun 10 m asl 41°23'01"S 072°16'23"W 5-Feb-05 Smicridea (S.) cf. annulicornis AN CL, Río del Este, ca. 3 km S Ralun 10 m asl 41°23'01"S 072°16'23"W 5-Feb-05 Smicridea (S.) cf. annulicornis AN CL, Río del Este, ca. 3 km S Ralun 10 m asl 41°23'01"S 072°16'23"W 5-Feb-05 Smicridea (S.) cf. annulicornis AN CL, Río del Este, ca. 3 km S Ralun 10 m asl 41°23'01"S 072°16'23"W 5-Feb-05 Smicridea (S.) cf. annulicornis AN CL, Río del Este, ca. 3 km S Ralun 10 m asl 41°23'01"S 072°16'23"W 5-Feb-05 Smicridea (S.) cf. annulicornis AN CL, Río Cochomó, 6 km NE bridge 40 m asl 41°27'26"S 072°13'06"W 4-Feb-05 Smicridea (S.) frequens AN CL, Rio Chaicas, ca 7km E of Lenca, just out PN Alerce Andino 130 m asl 41°35.644'S 72°35.610'W 11-Feb-08 Smicridea (S.) frequens AN CL, Rio Chaicas, ca 7km E of Lenca, just out PN Alerce Andino 130 m asl 41°35.644'S 72°35.610'W 11-Feb-08 Smicridea (S.) mucronata AN CL, Rio Chaicas, ca 7km E of Lenca, just out PN Alerce Andino 130 m asl 41°35.862'S 72°36.011'W 9-Feb-08 Smicridea (S.) annulicornis AN CL, Rio Chaicas, ca 7km E of Lenca, just out PN Alerce Andino 130 m asl 41°35.862'S 72°36.011'W 9-Feb-08 Smicridea (S.) annulicornis AN CL, Rio Chaicas, ca 7km E of Lenca, just out PN Alerce Andino 130 m asl 41°35.862'S 72°36.011'W 9-Feb-08 Smicridea (S.) annulicornis AN CL, Rio Chaicas, ca 7km E of Lenca, just out PN Alerce Andino 130 m asl 41°35.862'S 72°36.011'W 9-Feb-08 Smicridea (S.) annulicornis AN CL, Rio Chaicas, ca 7km E of Lenca, just out PN Alerce Andino 130 m asl 41°35.862'S 72°36.011'W 9-Feb-08 Smicridea (S.) annulicornis AN CL, Rio Chaicas, ca 7km E of Lenca, just out PN Alerce Andino 130 m asl 41°35.862'S 72°36.011'W 9-Feb-08 Smicridea (S.) annulicornis AN CL, Rio Chaicas, ca 7km E of Lenca, just out PN Alerce Andino 130 m asl 41°35.862'S 72°36.011'W 9-Feb-08 Smicridea (S.) cf. penai AN CL, Rio Chaicas, ca 7km E of Lenca, just out PN Alerce Andino 130 m asl 41°35.862'S 72°36.011'W 9-Feb-08 Smicridea (S.) mucronata CH CL, Isla de Chiloé, Río Verde, 1.9 km W of Puntra 40 m asl 42°07.078'S 73°50.364'W 3-Feb-05 Smicridea (S.) annulicornis CH CL, Isla de Chiloé, Río Verde, 1.9 km W of Puntra 40 m asl 42°07.078'S 73°50.364'W 3-Feb-05 Smicridea (S.) annulicornis CH CL, Isla de Chiloé, Río Verde, 1.9 km W of Puntra 40 m asl 42°07.078'S 73°50.364'W 3-Feb-05 Smicridea (S.) frequens CH CL, Isla de Chiloé, Río Verde, 1.9 km W of Puntra 40 m asl 42°07.078'S 73°50.364'W 3-Feb-05 Smicridea (S.) mucronata CH CL, Isla de Chiloé, Río Verde, 1.9 km W of Puntra 40 m asl 42°07.078'S 73°50.364'W 3-Feb-05 Smicridea (S.) mucronata CH CL, Isla de Chiloé, Río Verde, 1.9 km W of Puntra 40 m asl 42°07.078'S 73°50.364'W 3-Feb-05 Smicridea (S.) mucronata CH CL, Isla de Chiloé, Río Verde, 1.9 km W of Puntra 40 m asl 42°07.078'S 73°50.364'W 3-Feb-05 Smicridea (S.) mucronata CH CL, Isla de Chiloé, Río Verde, 1.9 km W of Puntra 40 m asl 42°07.078'S 73°50.364'W 3-Feb-05 Smicridea (S.) mucronata CH CL, Isla de Chiloé, Río Verde, 1.9 km W of Puntra 40 m asl 42°07.078'S 73°50.364'W 3-Feb-05 Smicridea (S.) mucronata CH CL, Isla de Chiloé, Río Verde, 1.9 km W of Puntra 40 m asl 42°07.078'S 73°50.364'W 3-Feb-05 Smicridea (S.) mucronata CH CL, Isla de Chiloé, Río Verde, 1.9 km W of Puntra 40 m asl 42°07.078'S 73°50.364'W 3-Feb-05 Smicridea (S.) mucronata CH CL, Isla de Chiloé, Río Verde, 1.9 km W of Puntra 40 m asl 42°07.078'S 73°50.364'W 3-Feb-05 Smicridea (S.) penai CH CL, Isla de Chiloé, Río Verde, 1.9 km W of Puntra 40 m asl 42°07.078'S 73°50.364'W 3-Feb-05 Smicridea (S.) penai CH CL, Isla de Chiloé, Río Verde, 1.9 km W of Puntra 40 m asl 42°07.078'S 73°50.364'W 3-Feb-05 Smicridea (S.) penai CH CL, Isla de Chiloé, Río Verde, 1.9 km W of Puntra 40 m asl 42°07.078'S 73°50.364'W 3-Feb-05 Smicridea (S.) penai CH CL, Isla de Chiloé, Río Verde, 1.9 km W of Puntra 40 m asl 42°07.078'S 73°50.364'W 3-Feb-05 Smicridea (S.) penai CH CL, Isla de Chiloé, Río Verde, 1.9 km W of Puntra 40 m asl 42°07.078'S 73°50.364'W 3-Feb-05 Smicridea (S.) penai CH CL, Isla de Chiloé, Río Verde, 1.9 km W of Puntra 40 m asl 42°07.078'S 73°50.364'W 3-Feb-05 Smicridea (S.) penai CH CL, Isla de Chiloé, Río Verde, 1.9 km W of Puntra 40 m asl 42°07.078'S 73°50.364'W 3-Feb-05 Smicridea (S.) smilodon * RJB: R.J. Blahnik; MLCL: M.L. Chamorro-Locayo; RWH: R.W. Holzenthal; HM: H. Malicky; PKM: P.K. Mendez; SUP: S.U. Pauls; FJMQ: F.J. Munoz-Quesada

Pauls et al_JNABS_Barcoding Chilean Smicridea_Appendix 1.xls

UMSP SpecimenID / BOLD sample ID UMSP000129927 UMSP000117585 UMSP000117587 UMSP000117589 UMSP000117590 UMSP000117591 UMSP000124980 UMSP000132430 UMSP000132431 UMSP000132432 UMSP000132433 UMSP000132442 UMSP000117586 UMSP000085471 UMSP000085472 UMSP000085473 UMSP000085474 UMSP000085475 UMSP000085476 UMSP000106708 UMSP000106709 UMSP000106710 UMSP000106711 UMSP000106712 UMSP000106713 UMSP000106714 UMSP000106715 UMSP000210823 UMSP000132429 UMSP000132428 UMSP000113505 UMSP000113506 UMSP000113507 UMSP000113508 UMSP000113509 UMSP000113510 UMSP000132334 UMSP000132335 UMSP000113525 UMSP000113526 UMSP000105566 UMSP000105572 UMSP000105573 UMSP000105575 UMSP000105576 UMSP000105577 UMSP000105578 UMSP000105580 UMSP000105581 UMSP000113518 UMSP000113519 UMSP000113520 UMSP000113522 UMSP000113523 UMSP000113527 UMSP000113528 UMSP000106950

Collector*

Stage

Haplotype

RWH, PKM, SUP RWH, RJB, MLCL RWH, RJB, MLCL RWH, RJB, MLCL RWH, RJB, MLCL RWH, RJB, MLCL RWH, PKM, SUP, MLCL RWH, PKM, SUP, MLCL RWH, PKM, SUP, MLCL RWH, PKM, SUP, MLCL RWH, PKM, SUP, MLCL RWH, PKM, SUP, MLCL RWH, RJB, MLCL RWH, RJB, MLCL RWH, RJB, MLCL RWH, RJB, MLCL RWH, RJB, MLCL RWH, RJB, MLCL RWH, RJB, MLCL RWH, RJB, MLCL RWH, RJB, MLCL RWH, RJB, MLCL RWH, RJB, MLCL RWH, RJB, MLCL RWH, RJB, MLCL RWH, RJB, MLCL RWH, RJB, MLCL RWH, RJB, MLCL MLCL, RWH, PKM, SUP MLCL, RWH, PKM, SUP MLCL, RWH, PKM, SUP MLCL, RWH, PKM, SUP MLCL, RWH, PKM, SUP MLCL, RWH, PKM, SUP MLCL, RWH, PKM, SUP MLCL, RWH, PKM, SUP MLCL, RWH, PKM, SUP MLCL, RWH, PKM, SUP RWH, RJB, MLCL RWH, RJB, MLCL RWH, RJB, MLCL RWH, RJB, MLCL RWH, RJB, MLCL RWH, RJB, MLCL RWH, RJB, MLCL RWH, RJB, MLCL RWH, RJB, MLCL RWH, RJB, MLCL RWH, RJB, MLCL RWH, RJB, MLCL RWH, RJB, MLCL RWH, RJB, MLCL RWH, RJB, MLCL RWH, RJB, MLCL RWH, RJB, MLCL RWH, RJB, MLCL RWH, RJB, MLCL

Female Male Male Male Male Male Male Male Male Male Male Female Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Female Male Larva Larva Larva Larva Larva Larva Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Male Female Female Male

Hap078 Hap058 Hap058 Hap058 Hap058 Hap058 Hap058 Hap048 Hap047 Hap047 Hap046 n111 n138 Hap046 Hap046 Hap046 Hap046 Hap047 Hap048 Hap085 Hap007 Hap085 Hap007 Hap085 Hap085 Hap007 Hap007 Hap020 n106 Hap047 Hap009 Hap011 Hap009 Hap009 Hap009 Hap009 Hap058 Hap047 n140 Hap013 Hap019 Hap047 Hap047 Hap047 Hap050 Hap047 Hap047 Hap047 Hap047 Hap060 Hap061 Hap060 Hap062 Hap060 Hap062 Hap060 Hap084

GenBank Sequence Accession Origin HM065446 B HM065213 B HM065214 B HM065215 B HM065216 B HM065217 B HM065218 B HM065303 MN HM065304 MN HM065305 MN HM065306 MN HM065397 MN HM065065 B HM065318 B HM065319 B HM065320 B HM065321 B HM065322 B HM065323 B HM065177 B HM065128 B HM065129 B HM065130 B HM065131 B HM065132 B HM065133 B HM065134 B HM065268 B HM065248 MN HM065302 MN HM065073 B HM065074 B HM065075 B HM065076 B HM065077 B HM065078 B HM065211 MN HM065301 MN HM065066 B HM065067 B HM065263 B HM065356 B HM065357 B HM065358 B HM065359 B HM065360 B HM065361 B HM065362 B HM065363 MN HM065399 B HM065400 B HM065401 B HM065402 B HM065403 B HM065404 B HM065405 B HM065483 B

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