2nd Global Webinar on

DIABETES AND ENDOCRINOLOGY November 28-29, 2022 | GMT+1

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SCIENTIFIC PROGRAM

2nd Global Webinar on Diabetes and Endocrinology 4

Day 1: November 28, 2022 Introduction: 08:40-09:00 ORAL PRESENTATION 09:00-09:25

Application of a pedometer for the management of impaired glucose tolerance in pregnant women Mariko Ueno, Tokyo Women's Medical University, Japan

09:25-09:50

The association between alcohol intake and obesity in a sample of the Irish adult population Salma Alkalbani, Family physician, Oman

09:50-10:15

The significance of Transforming Growth Factor-Beta (TGF-β) in topical ADMSC-CM application for chronic plantar ulcer of leprosy: A randomized controlled trial Medhi Denisa Alinda, Universitas Airlangga-Dr. Soetomo General Academic Hospital, Indonesia

10:15-10:40

Predicted HbA1c and fructosaminated HbA1c: Evaluating their role as an indicator of glycemic status in diabetes mellitus: A hospital based cross sectional study Montosh Chakraborty, AIIMS, India

10:40-11:05

A systematic approach to patient with cushing’s syndrome Gaye Didem Cabukoglu, Cukurova University, Turkey

11:05-11:30

Clinical features and biochemistry at onset of diabetes from the baseline data of registry of young people with diabetes in paediatric diabetes center in BIRDEM, Dhaka, Bangladesh Kishwar Azad, BADAS Perinatal Care Project, Bangladesh

11:30-11:55

Hypertensive phenotypes and pattern of ambulatory blood pressure in patients of diabetes mellitus of Kashmir valley Mohammad Rafi Mir, Physician Health Services, India

11:55-12:20

Levels of vitamin D and their association with complications of type 2 diabetes mellitus Sukhraj Kaur, Government Medical College, India Lunch (12:20-12:50)

12:50-13:15

Anatomic and topographic vitreous and vitreoretinal interface features during chromovitrectomy of A,B,C stages of proliferative diabetic vitreoretinopathy Natalia Kislitsyna, The S. Fyodorov Eye Microsurgery Federal State Institution, Russia

13:15-13:40

Long term Sustained growth of insulin producing cells using agarose-chitosan coated silver nanocomposites Anasuya Ganguly, BITS Pilani, India

13:40-14:05

Virtual screening for chemical analogues similar to phytochemicals that inhibit aldose reductase in the development of diabetic microvascular complications Filex Otieno, Kabarak University, Kenya

2nd Global Webinar on Diabetes and Endocrinology 5

SCIENTIFIC PROGRAM

14:05-14:30

The pathogenetic significance of endothelial dysfunction in the development of vascular diabetic complications Elena Reznikova, Volgograd Medical State University, Russia

14:30-14:55

Behavioral factors of diabetes: The experience of a pilot study Aminat Magomedova, Moscow State University, Russia

14:55-15:20

Deficient autophagy contributes to the development of diabetic retinopathy Jacqueline M Lopes de Faria, University of Campinas, Brazil

15:20-15:45

Evaluate the consumption of protein of animal-source in complementary foods of offspring of mothers with gestational diabetes in an educational intervention: 18-month follow-up Sandra Lorena Garcia Del Rio, Instituto Mexicano del Seguro Social, Mexico

15:45-16:10

Elevated procalcitonin levels in a patient with diabetic ketoacidosis in the absence of infection Basheer Mohammed, Texas Tech University Health Sciences Center, USA

16:10-16:35

Cardiovascular outcomes in different gender and ethnicities treated with SGLT2 inhibitors and GLP-1 receptor agonists: A systematic review and meta-analysis of randomized trial Andrey Manov, Touro University College of Medicine, USA Day 1 concludes Day 2: November 29, 2022 Introduction: 08:30-08:45 ORAL PRESENTATION

08:45-09:00

To assess the risk factors for the presence and severity of Obstructive Sleep Apnea (OSA) among patients with Type 2 Diabetes Mellitus (T2DM) Divij Sharma, St John of God Hospital, Australia

09:00-09:25

Biopolymer grafted silver nanofibre for the treatment of diabetic foot ulcer Rebika Baruah, CSIR-North East Institute of Science and Technology, India

09:25-09:50

Urinary levels of podocalyxin as a marker for podocytopathy in patients with metabolic syndrome having high body mass index: A diagnostic test accuracy study Ammar Y Neanaa, Main University Hospitals, Egypt

09:50-10:15

Effect of short duration moderate intensity physical activity on glycemic control and antioxidant status of prediabetic population Zubia Shah, Khyber Girls Medical College, Pakistan

10:15-10:40

Molecular basis of pancreatitis Asma Ahmed, The University of Lahore, Pakistan

10:40-11:05

Effect of raja yoga meditation on glycaemic status in type 2 diabetes mellitus" Nitu Nigam, K.G’s Medical University, India

2nd Global Webinar on Diabetes and Endocrinology

SCIENTIFIC PROGRAM

11:05-11:30

A specific coagulation disorder of uncommon cause Hridya Harimohan, Kerala Institute of Medical Sciences, India

11:30-11:55

Sleep quality on diabetes: A pilot study Rita Aguiar Fonseca, ACES Baixo Mondego, Portugal

11:55-12:20

Type 2 diabetic women with asymptomatic bacteriuria: A crosssectional study Meghana B S, Victoria hospital, India Lunch (12:20-12:50)

12:50-13:15

Autoimmune diseases accompanying hashimoto thyroiditis in pediatric patients Gizem Gencan, Yozgat Bozok University Research and Application Hospital, Turkey

13:15-13:40

Awareness of diabetic retinopathy among Saudis with diabetes type 2 in Riyadh city Yousef Mohammed Alluhaymid, King Saud University, KSA

13:40-14:05

The hidden impact of diabetes on sexual function: A pilot study Rita Aguiar Fonseca, ACES Baixo Mondego, Portugal

14:05-14:30

Impact of digital world food marketing on attitude and practices associated with unhealthy food intake causing metabolic syndrome risk factors in adolescents Anshoo Agarwal, Northern Border University, KSA

14:30-14:55

Educational interventions in type 2 diabetes mellitus patients in primary health care: Systematic review and meta-analysis Yousef Mohammed Alluhaymid, King Saud University, KSA

14:55-15:20

Burnout: Analysis and intervention on a medical community Rita Aguiar Fonseca, ACES Baixo Mondego, Portugal

15:20-15:45

Breast cancer in men: A case of study Daisy Alejandra Morales-Lazcano, Instituto Mexicano del Seguro Social, Mexico

15:45-16:10

The IGSF1 deficiency syndrome may present with normal free t4 levels, severe obesity, or premature testicular growth Steven Ghanny, Hackensack University Medical Center, USA E-poster

16:10-16:20

Phaeochromocytoma induced takotsubo cardiomyopathy Erin Wharemate, Auckland District Health Board, New Zealand Day 2 concludes

2nd Global Webinar on Diabetes and Endocrinology

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ORAL

PRESENTATION

DAY- 01

Diabetes 2022 9

ORAL SPEAKERS

Application of a pedometer for the management of impaired glucose tolerance in pregnant women Ueno Mariko Tokyo Women's Medical University, Japan

Abstract:

T

he proper management of Impaired Glucose Tolerance (IGT) in pregnant women has a great impact on the prognoses of both the mother and the infant and is of interest to both obstetricians and diabetologists. Although nutritional intervention along with insulin treatment in the mainstream approach of IGT treatment in pregnant women, exercise intervention is another important component of the IGT management. One explanation why the effect of exercise for glucose metabolism is controversial might be the problems with quantifying the amount of exercise. Thus, we used pedometers to quantitatively assess the amount of exercise performed by pregnant women with IGT. A pedometer is a useful tool for objective exercise evaluation. With the widespread use of smartphones equipped with pedometer function, exercise by walking is easily monitored and utilized in both healthy pregnant women and pregnant women with obesity and IGT. Although it might be difficult to prove the effect of pedometer for the non-pregnant patients due to the problem with their compliance, we succeeded in proving the effect of exercise therapy with using pedometers for pregnant women with IGT. We present the present perspective on the use of a pedometer in the management of IGT in pregnant women by introducing our recently published work.

Biography: Mariko Ueno has graduated faculty of medicine, Tokyo Women’s Medical University, Japan in 2008 and she has completed her PhD from same university in 2020. She became a medical specialist of obstetrics and gynecology in 2013 and a medical specialist of perinatal and neonatal medicine in 2017. She has been an assistant professor of departments of obstetrics and gynecology, Adachi Medical Center, Tokyo Women’s Medical University, Japan since 2016.

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ORAL SPEAKERS

The association between alcohol intake and obesity in a sample of the Irish adult population Salma Alkalbani Family physician, Oman

Abstract: Background: The prevalence of obesity is increasing worldwide. Alcohol has been studied as a possible risk factor for obesity, but the evidence is discordant. This study examined the association between alcohol consumption and obesity in an Irish population. Method: A cross-sectional study using secondary data from the healthy Ireland survey 2017 was conducted. Descriptive and comparative data were analysed to identify associations of alcohol-related variables with Waist Circumference (WC) and Body Mass Index (BMI). Regression analysis was performed to examine the associations between harmful alcohol consumption (AUDIT-C score≥ 5) and obesity indicators. Adjustments were made for sociodemographic variables, health-related variables and other alcohol-related variables. Result: A total of 7486 participants took part in this survey (response rate=60.4%). Most of the participants (86%) were alcohol drinkers, with the majority drinking less than 3 times per week (77.5%); 49.1% were considered harmful drinkers. After controlling for possible confounders, positive associations of harmful alcohol consumption with WC (β=1.978, 95% CI: 0.996, 2.960) and BMI (OR=1.247, 95% CI: 1.058, 1.471) were observed. Further controlling for alcohol consumption frequency and binge drinking made this association nonsignificant. Unlike less frequent binge drinking, frequent binge drinking was positively associated with WC. Conclusion: Harmful alcohol consumption was associated with obesity (high BMI, large WC) after controlling for possible confounders; However, this association became nonsignificant after controlling for other alcohol-related variables. Frequent binge drinkers were more likely to have a large WC. Further longitudinal studies to examine the exact association between alcohol consumption and obesity are warranted.

Biography: Salma Alkalbani is an Omani family physician. Her credentials include an MD, MRCGP, Arab Bard, MPH and a diplomate MFPHM. She obtained her family medicine board certification from the Oman Medical Specialty Board in 2014. In 2021, She received her master's degree in public health with honours from the University College Dublin. She is a diplomate member of the faculty of public health medicine, Ireland. Currently, she is doing her clinical fellowship in public health in Ireland. Her research areas of interest are obesity, diabetes and tobacco use through health promotion and prevention. She served as an editorial member for several journals.

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ORAL SPEAKERS

The significance of Transforming Growth Factor-Beta (TGF-β) in topical ADMSC-CM application for chronic plantar ulcer of leprosy: A randomized controlled trial Medhi Denisa Alinda Universitas Airlangga-Dr. Soetomo General Academic Hospital, Indonesia

Abstract:

C

hronic wounds are characterized by the inability to heal in an expected time frame, often related to defects in the orchestrated wound healing to reepithelize over recovered dermal tissue. The secretome of adipose Mesenchymal Stem Cell-Conditioned Medium (ADMSC-CM) produces growth factors (i.e., Transforming Growth Factor [TGF]) and cytokines in the hope of creating a favourable wound healing milieu, including in Chronic Plantar Ulcer of Leprosy (CPUL). This randomized controlled trial aims to compare the TGF-β between topical ADMSC-CM group (n = 16) and Farmazertin Gauze Dressing (FGD) only group (n = 16) on the healing of CPUL. The intervention was applied every three days for up to eight weeks. Ulcer size, adverse reactions and possible complications were monitored weekly. TGF-β was evaluated pre- and post-intervention through immunohistochemistry examination obtained from ulcer area and tissue and was compared between groups. In both groups, the healing percentage of the ulcer increased each week. Significant statistical differences (p-value < 0.05) in ulcer mean size and depth reduction were detected between groups from week 2 and 3, respectively. However, comparative mean TGF-β ± SD between groups showed no significant statistical difference (9.62 ± 4.53 vs 7.63 ± 4.27, p-value 0.209) with elevated TGF-β compared to pre- and post-intervention within each group (ADMSC-CM group: 6.81 ± 3.49 vs 9.62 ± 4.53; FGD only group: 6.38 ± 3.48 vs 7.63 ± 4.27). No adverse reactions or complications were noted. Topical ADMSC-CM is a novel therapeutic agent for targeting and correcting the microenvironment milieu due to prolonged inflammation in a chronic wound, including CPUL. TGF-β is a crucial growth factor for wound healing. However, TGF-β may exert a biphasic, dose-dependent effect on MSC, potentially explaining the contradictory results between TGF-β and mesenchymal stem cell proliferation.

Biography: Medhi Denisa Alinda is a dermatovenereologist who completed her doctoral program at the age of 37 years old from Airlangga University, Indonesia. She is the head of the medical staff of Airlangga University Hospital and a lecturer at Dr. Soetomo General Academic Hospital, Indonesia. She has actively published and researched in the field of tropical disease, specifically leprosy and has been involved in international publication and collaborative research with institutions from London, India, Brazil, etc.

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ORAL SPEAKERS

Predicted HbA1c and fructosaminated HbA1c: Evaluating their role as an indicator of glycemic status in diabetes mellitus: A hospital based cross sectional study Montosh Chakraborty AIIMS, India

Abstract: Background: Glycated haemoglobin has been a popular investigation to access the glycemic status. It is a marker for intracellular glycation and hence an investigation which can comply with both intracellular and extracellular glycation status is preferred. Methodology: In this regard, Fructosaminated HbA1c (FHbA1c), Predicted HbA1c (PHbA1c) and Glycation Gap (GG) were evaluated in 57 cases of Diabetes Mellitus (DM) without complication and having a normal serum protein and albumin levels. 57 controls were also evaluated. FHbA1c, PHbA1c and Post prandial Predicted HbA1c (PPHbA1c) were plotted using population linear regression equation and calculated individually against each subject. The statistical calculation was evaluated using SPSS version 21. Results: FHbA1c, PHbA1c, PPHbA1c and GG were found to be significantly elevated in cases (p value < 0.001). FHbA1c and GG shows significant correlation with the glycemic indices in cases compared to controls. The correlation with FBS and PPBS increases as we move from controls to cases. Area Under Curve (AUC) in case of FHbA1c is 96.8% and cutoff level of 5.85% can result in sensitivity of 88.2% and specificity of 90% for the diagnosis of DM. Conclusion: FHbA1c can be used as an additional investigation in cases of DM. Along with GG, it is increased in DM and correlates with the glycemic indices. It has a decent AUC on plotting ROC. Thus, FHbA1c and GG can be used as a part of Glyco-metabolic profile and can provide insight into the individualised glycation tendency of the cell.

Biography: Montosh Chakraborty has completed his MBBS MD (Biochemistry) from a premier medical college in India. He has over 10 years of experience in the relevant field as academician, laboratory specialist and researcher. Presently he is working as faculty in All India Institute of Medical Sciences. He has many publications in high end journals and has been contributing as a reviewer for many journals. He has over 187 citations and h index is 8.

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ORAL SPEAKERS

A systematic approach to patient with cushing’s syndrome Gaye Didem Cabukoglu Cukurova University, Turkey

Abstract:

C

ushing's syndrome results from chronic exposure to excessive glucocorticoids. It may be caused by excessive ACTH production, usually by a pituitary corticotropic adenoma (Cushing's disease), less frequently by an extra pituitary tumour (ectopic ACTH syndrome), or very rarely by a tumour secreting of corticotropin-releasing hormone (ectopic CRH syndrome). Cushing’s syndrome can also be ACTH-independent when it results from cortisol-producing adrenal tumour, which may be benign or malignant; or by bilateral adrenal hyperplasia or dysplasia. Typical signs and symptoms of hypercortisolism, such as plethora, buffalo hump and truncal obesity, easy bruising, deep purple striae, proximal myopathy, acne, hirsutism, osteoporosis and glucose intolerance are observed in this syndrome. It is more common among patients with diabetes, obesity, hypertension and osteoporosis. This case emphasizes the need for systematic approach to patients presenting hypertension in terms of Cushing’s syndrome. A 20 years old woman presented our clinic due to hypertension. Blood pressure was 200/110 mmHg in her controls. Headache, weight gain, hirsutism was in her history. She had a moon face and menstrual irregularities. Examination showed that purple striae were in her extremities. She was referred to the endocrinology department for suspected Cushing’s syndrome. Laboratory analyses showed that increased cortisol (26 µg/dL, normal 5–15 µg/dL) and adrenocorticotropic hormone (ACTH:70 pg/mL, normal 9–52 pg/mL). 24 hour urine cortisol measurement exceeds 100 μg (1118 μg). When low-dose dexamethasone suppression test (1 mg overnight test) was taken, cortisol level increased (30 µg/dL). Magnetic resonance imaging of the pituitary showed that a tumour in the right side of the adenohypophysis. Diameter of the lesion was >10mm (pituitary macroadenoma). Visual field test was documented by the partial deficits of inferior and temporal quadrants of the visual field in the left eye. Computed tomography scans showed enlarged right adrenal gland. As a result of all examinations patient was diagnosed with Cushing’s Syndrome. Transsfenoidal surgery was planned and treatment was explained to the patient.

Biography: Gaye Didem Cabukoglu studied medicine faculty and graduated from Mustafa Kemal University, Turkey. She had a participant in IMFMC 22019, Spain Barcelona with "Correlates of patients\' satisfaction with physician visit to patients’ rational use of drug”, oral presentation, september 2019. She wrote an article “The biopsychosocial approach of illnesses in primary care’’ chapter in book ‘The new approaches in family medicine practice Episode:4’. She is a research assistant in Marmara University Faculty of Medicine department of family medicine, Istanbul, Turkey.

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ORAL SPEAKERS

Clinical features and biochemistry at onset of diabetes from the baseline data of registry of young people with diabetes in paediatric diabetes center in BIRDEM, Dhaka, Bangladesh Kishwar Azad BADAS Perinatal Care Project, Bangladesh

Abstract: Background: BADAS has developed a sustainable model of healthcare for people with or at risk for diabetes in Bangladesh. We here report the demographic, clinical and biochemical profile of the patients enrolled in the paediatric diabetes center registry of people with diabetes in Bangladesh. Methods: The paediatric diabetes center registry recruits all diabetes cases (newly diagnosed or treated) reporting on or after 1 January 2010 with age of diagnosis ≤26 years. A retrospective medical record review of young people with diabetes in Paediatric diabetes center over a period (January 2010 to December 2015) was done. A baseline proforma was used to obtain information on demographic, clinical and laboratory details at registration. Results: The registry has enrolled 2235 patients, majority (54.5%) were female and 43.8% were male; with T1DM (87.0%; n = 1945), T2DM (4.6%; n = 102) and other types of DM (8.4%; 188). The mean (SD) age at diagnosis for T1DM was 11.7 ±3.7 years, while that for T2DM was 12.7 ±2.3years and for other types was11.9 ±4.3) years. Majority of the T1 DM were from rural area whereas T2 D were from mostly urban area(P=.0001). A large proportion of T2 DM were from high socioeconomic status (p.0001) and had higher BMI compared to T1DM (p.0001). Mean HbA1c was higher in T1D (12.4 ±3.3) compared to T2 D ( 10.3. ± 3.0) (p .0001). Conclusion: These data offer insights into the demographic and clinical characteristics of diabetes in youth in Bangladesh. Further research is needed to better understand the natural history of the different types of diabetes.

Biography: Kishwar Azad is a project director of Perinatal Care Project (PCP) which is a collaboration between centre of global health at University College London and Diabetic Association of Bangladesh. He was trained as a paediatrician, his interests were in neonatology and diabetes in children and adolescents. However, in 2006 he moved to PCP and became engaged in research into the role of community mobilisation and maternal and neonatal health and later diabetes.

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ORAL SPEAKERS

Hypertensive phenotypes and pattern of ambulatory blood pressure in patients of diabetes mellitus of Kashmir valley Mohammad Rafi Mir Physician Health Services, India

Abstract:

S

ome patients of Diabetes Mellitus (DM) who are normotensive or have controlled Blood Pressure (BP) on Office BP Measurement (OBPM) may be having hypertension or uncontrolled blood pressure on Ambulatory Blood Pressure Monitoring (ABPM). We conducted an observational study to compare OBPM with ABPM and thus determine hypertensive phenotypes like Masked Hypertension (MH) and white coat hypertension and pattern of blood pressure in diabetic patients of our population. Two hundred patients of DM with or without hypertension were included in this study, of whom 32 were normotensives, 46 were hypertensives controlled on anti-hypertensive treatment, 22 were hypertensives not on anti hypertensive treatment and 100 were hypertensives uncontrolled on anti hypertensive treatment. Among 32 normotensive diabetics, 17 (53%) patients had MH on ABPM. Out of these 32 normotensive patients, 7 (21.8%) had isolated nocturnal hypertension, 3 (9.3%) had isolated day time hypertension and 7 (21.8%) had day time and nocturnal hypertension. Non dipping pattern was found in 53% of patients of masked hypertension. Out of 46 hypertensive diabetics with controlled OBPM on anti-hypertensive treatment, 26 (56.5%) had masked uncontrolled hypertension on ABPM. Out of 22 diabetics with treatment naive hypertension, 7 (32%) were found to have white coat hypertension on ABPM. Fifteen (15%) patients out of 100 hypertensive diabetics with uncontrolled OBPM despite on anti hypertensive were found to have white coat effect on ABPM. Non dipping pattern was significantly associated with longer duration of diabetes (≥120 months), retinopathy and neuropathy. To rely exclusively on OBPM to diagnose hypertension and monitor blood pressure may underestimate the CVD risk especially in diabetics. ABPM is a tool that may help clinicians in starting anti-hypertensive treatment appropriately, avoiding unnecessary anti hypertensive treatment and/or withdrawing anti hypertensive treatment as indicated.

Biography: Mohammad Rafi Mir completed his M.D in internal medicine from Sher-I-Kashmir Institute of Medical Sciences in 2020 and is presently working as a primary care physician in Directorate of Health Services Kashmir.

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ORAL SPEAKERS

Levels of vitamin D and their association with complications of type 2 diabetes mellitus Sukhraj Kaur Government Medical College, India

Abstract: Introduction: Type 2 diabetes mellitus is a non-communicable disorder associated with hyperglycaemia. It is associated with various micro and macro vascular complications. Vitamin D a stercosterol is an important molecule associated with many skeletal and extra skeletal functions in the body. Vitamin D maintains glucose homeostasis and has an important role to play in manifestations of various complications. Material and Method: The present study comprised of one hundred patients of diabetic complications compared with one hundred normal individuals. All the subjects were investigated for blood glucose, glycated haemoglobin, lipid profile and vitamin D. Results: Glycemic control and lipid profile was deranged in patients with complications of type 2 diabetes mellitus. Levels of vitamin D were significantly decreased in patients as compared to controls. Moreover 73% of the patients were deficient in vitamin D. Conclusions: Vitamin D acts through various non skeletal pathways and helps to regulate glucose. In the current study it showed negative correlation with glycated haemoglobin, triglycerides and cholesterol thus indicating that maintaining vitamin D levels can delay the onset of diabetic complications.

Biography: Sukhraj Kaur Dhillon was born in the year 1968 and she did her post-graduation in biochemistry with distinction and is a gold medallist in her subject. She joined the current assignment in the year 1991 and is currently working as assistant professor and head biochemistry at Government Medical College, Amritsar, Punjab (India), where she is teaching biochemistry to undergraduates and post-graduates, guides postgraduate and doctoral students in their research projects. She has completed her PhD research project on ‘diabetes mellitus’ (pertaining to lifestyle modifications). She has to her credit, 30 national and international publications in journals of good standing and repute. She has presented her research work in various national and international conferences and has won numerous awards and recognitions. Her research interests include diabetes and its complications, thyroid dysfunction and coronary artery disease. Presently she is working on an ICMR project related to diabetes. Her passion of teaching made her a keen learner also which is evident from the fact that she did her advanced course in medical education and is fulfilling the responsibility of member of medical education unit of her Institute. Apart from being an avid researcher and a disciplined teacher, she is kind hearted, encourages her students to interact with her for clarification of their doubts. She is god fearing and a lover of art.

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ORAL SPEAKERS

The pathogenetic significance of endothelial dysfunction in the development of vascular diabetic complications Natalia Kislitsyna The S. Fyodorov Eye Microsurgery Federal State Institution, Russia

Abstract: Resume: The role of the vitreous body and Vitreomacular Interface (VMI) is key in many processes including Proliferative Diabetic Retinopathy (PDR). In PDR patients, the VMI changes can significantly influence the emergence and progression of the disease. There are multiple factors at work in the VMI including taut posterior cortical layers, vitreoschisis, Posterior Vitreous Detachment (PVD) and vitreous adhesions. But there is no general consensus about their role in proliferative complications. Further understanding the VMI relationship in a case of PDR is warranted in order to design better treatments, to arrest and possibly even reverse progression of PDR. Today there is no imaging techniques to determine normal vitreous and VMI interactions in different PDR stages intraoperatively. Purpose: To analyze intraoperative vitreous and vitreoretinal interface features during chromo vitrectomy in patients with A-C stages of PDR. Materials and Methods: Seventy-four diabetic patients (74 eyes) were included. We performed standard 25 Gauge pars plana vitrectomy using Vitreo contrast for vitreous and Vitreoretinal Interface (VRI) visualization. Intravitreal «Vitreo contrast» suspension is the most favored agent of those studied and it is increasingly used as an adjunct during surgery to delaminate fine tissue planes and pockets of formed vitreous and VRI structures that may not be visible with routine operative illumination systems, or using modern vital dyes. Results: «Vitreo contrast» suspension allows to visualize posterior cortex changes during different stages of PDR. We investigated vitreous and VRI anatomy, topography and structure and determined safety of retrociliary and equatorial cisterns walls in 97% in stage A of PDR, 95% in stage B and in 82% of stage C. In 3-5-18% cases, correspondently, we determined disorganization of some vitreous cisterns. In 94 % cases of PDR A and 96 % cases of PDR B we visualized preretinal vitreous layer in a central macular zone, within the boundaries of vascular arcades. It has specific topography and strong adhesion to the internal retinal membrane. It’s the first time when this new vitreous cortex layer was revealed. The presence of this layer is the result of a strong vitreomacular adhesion that causes the posterior vitreous cortex split as it attempts to detach from the inner retinal surface. Such outermost layer remains attached to the macula and can induce further proliferation process. On a stage B of PDR this area corresponds with multiple vitreoschisis, on a stage C of PDR – with fibrovascular membrane. The complete PVD was revealed in 61 cases. Conclusion: In this article we analyze the results of surgical treatment of 74 patients with A-C stages of proliferative diabetic retinopathy. Newer imaging technique with new dye - suspension «Vitreocontrast» allows to detect sensitive relationships of vitreous and VRI in each stage of the disease. The role of vitreous body in this process gives us a reason to consider it as an important object for further research. Moreover, the understanding of their relations in different stages of PDR enables to develop optimal surgical approach on each stage of PDR.

Biography: At the age of 30, she defended her dissertation on the topic "Surgical treatment of the consequences of penetrating wounds, taking into account the data of ultrasound biomicroscopy". For 20 years she worked as an ophthalmic surgeon in the department of vitreoretinal surgery. Author of more than 70 publications. Member of the European Society of Vitreoretinal Surgeons. Currently director of the Institute of Continuing Professional Education S. Fyodorov Eye Microsurgery Federal State Institution, Moscow.

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ORAL SPEAKERS

Long term Sustained growth of insulin producing cells using agarose-chitosan coated silver nanocomposites Anasuya Ganguly BITS Pilani, India

Abstract

D

iabetes is a group of diseases characterized by high levels of blood sugar for an extended period. Despite newer and effective therapy, current treatment is riddled with fundamental challenges. To overcome the adverse effects of existing drugs, regenerative medicine has emerged as an essential treatment, for which tissue engineering may serve as a foundation for the repair of pancreatic cells secreting insulin. Different polymeric scaffolds have been explored for pancreatic tissue engineering. In the current study, a continuation of our preceding work we attempt to test the role of previously synthesized agarose-chitosan coated silver nanocomposite scaffold (AG-CHNp) for the long-term growth of pancreatic cells. Pancreatic cells were isolated from BALB/c mice and were characterized by dithizone (DTZ) staining, real time polymerase chain reaction (RT-PCR), western blotting, and flow cytometry for characteristic pancreatic markers. The isolated population of cells was grown on scaffolds and its effectiveness towards insulin secretion was studied. The isolated population was found to be positive for glucagon, PDX-1 and Pax-4, while a 200-fold change transcript level of insulin was observed. The cells upon seeding on the scaffolds exhibited sustained growth and insulin secretion as confirmed by western blotting. Overall, the study demonstrates the suitability and application of AG-CHNp for pancreatic tissue engineering.

Biography Anasuya Ganguly completed her PhD at the age of 31 years from Jadavpur University and Bose Institute, Kolkata, India. She is an associate professor at the department of biological sciences, BITS-Pilani, K K Birla Goa Campus, India. She has over 31 publications that have been cited over 509 times and his publication h-index is 13. She has been serving as an editorial board member of several reputed journals.

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ORAL SPEAKERS

Virtual screening for chemical analogues similar to phytochemicals that inhibit aldose reductase in the development of diabetic microvascular complications Filex Otieno Kabarak University, Kenya

Abstract: Background: The pathological hallmark in diabetic complications surrounds the vasculature system causing microvascular and macrovascular complications to develop. Such development follows a complex pathophysiology with inputs from several cellular biochemical pathways; One of them being the polyol pathway. Herbal extracts have been shown to impede polyol pathway by inhibiting aldose reductase; the first and rate limiting step in the two-step reaction. Such extracts offer an alternative modality for slowing onset and progression of microvascular complications in diabetic patients. Flavonoids in particular have been studied and in repeated accounts shown to have even stronger inhibitory activity. This study aimed at assessing analogy of specific flavonoids that delay onset of microvascular complications with better pharmacokinetic and toxicology profile. Methodology: An in-silico study design was employed. The phytochemicals-luteolin and quercetin were selected. Analogy was obtained from ZINC database and prepared using Avogadro software. Docking analysis was done using AutoDock Vina embedded in Chimera. Ligand enzyme interaction was carried out using Biovia Discovery studio. Pharmacokinetic and toxicological profiling was carried out using SWISSADME and protox server. A total of 40 analogues constituting 20 ZINC compounds for each phytochemical were analyzed. Sulindac was used as the comparator besides the original phytochemicals. Results: Docking analysis showed both luteolin and quercetin (-9.7) had a slightly stronger affinity for inhibiting aldose reductase compared to Sulindac (-9.6). Further, 8 analogues of luteolin and 14 analogues of quercetin had docking scores more negative than both parent phytochemicals and Sulindac with strongest affinity registered at -10.6. pharmacokinetic modelling of sulindac showed it did not violate the Lipinski rule, had a high GI absorption, neither penetrated BBB nor acted upon by p-glycoprotein and inhibited CYP-2C19, -2C9 and -3A4. However, the comparator had a low LD50 (264mg/ kg). Comparatively, both luteolin and quercetin did not violate Lipinski rule, had high GI absorption, did not cross BBB nor were they P-gp substrate and inhibited CYP1A2, CYP2D6 and CYP3A4. The LD50 of luteolin (3919mg/kg) was very high indicating excellent safety profile. Quercetin however, had a very low LD50 (159mg/kg). All 22 analogues exhibited similar pharmacokinetic profile to their respective phytochemical. However, they did differ in terms of docking strength and toxicology analysis. Six out of the 8 luteolin analogues had LD50=3919mg/kg while remaining two compounds had LD50=159mg/kg. Five quercetin analogues had LD50 of 159mg/kg while another five had LD50=3919mg/kg. Two of the remaining four quercetin analogues had LD50=4000mg/kg while the other two had a LD50 of 5000mg/kg. Conclusion: In conclusion 6 ZINC compounds similar to luteolin and 9 similar to quercetin had stronger binding affinity for aldose reductase and superior toxicological profile compared to parent phytochemicals.

Biography: Filex Otieno is currently an undergraduate finalist student pursuing a bachelor of pharmacy degree at Kabarak University. He has several publications including research articles, a book and book chapter. He also serves as an editorial board member and as a copy editor in several journals. His research interests lie within the fields of molecular immunology and pharmacology.

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ORAL SPEAKERS

The pathogenetic significance of endothelial dysfunction in the development of vascular diabetic complications Elena Reznikova Volgograd Medical State University, Russia

Abstract:

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ne important mechanism for the progression of long-term complications in patients with Diabetes Mellitus (DM) is currently considered an impaired function of the vascular endothelium. The presence of endothelial imbalance indicates the involvement of the vasculature in a vicious circle of metabolic consequences of hyperglycaemia and also reflects the severity and pervasiveness of this process. At patients with DM found a significant increase in the level of endothelin-1 and von Willebrand factor (ffW) compared with the control group of healthy individuals, patients with arterial hypertension and patients without diabetic angiopathy. In the presence of vascular complications of DM, an increased level of endothelin-1 was found in 79.4% of patients, ffW in 65.4%, reduced level of endothelial NO-synthase (e-NOS) in 66.4% sick. The most significant increase in the level of endothelin-1 and ffW is associated with a long course DM. The criterion for insufficient or unsatisfactory glycemic control is increased levels of endothelin-1. Study of circulating markers of endothelial dysfunction can be used as a method of assessing the potential risk of vascular damage and for the purpose of diagnosing vascular diabetic complications.

Biography: Elena Reznikova has completed her PhD at the age of 38 years from Volgograd State Medical University, Russia. She is an assistant professor at faculty therapy department of VSMU, Russia. She has over 50 publications in different medical journals. She has been performing as a high-qualified endocrinologist for more than 15 years.

2nd Global Webinar on Diabetes and Endocrinology 21

ORAL SPEAKERS

Behavioral factors of diabetes: The experience of a pilot study Aminat Magomedova Moscow State University, Russia

Abstract:

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t is more reasonable to direct the main efforts in the fight against diabetes to its prevention. The report will provide an overview of the behavioural features of adolescents that affect the development of diabetes, based on a pilot study conducted by the author and substantiates the need for an interdisciplinary approach and consolidation of efforts in the fight against NCDs in the 21st century. An innovative device that allows people to control eating behaviour will be presented.

Biography: Aminat Magomedova graduated from Lomonosov MSU in 1994, received a PhD at the age of 28. Author of 9 books, 20 articles. Laureate of the MSU Prize for achievements in teaching and methodological work. Received the gratitude of the head of the Republic of Dagestan for scientific, educational and organizational work during the covid-19 pandemic. Mother of 5 children.

2nd Global Webinar on Diabetes and Endocrinology 22

ORAL SPEAKERS

Deficient autophagy contributes to the development of diabetic retinopathy Jacqueline M Lopes de Faria University of Campinas, Brazil

Abstract:

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utophagy is a self-degradation process essential to maintain intracellular homeostasis and cell survival, controlling pathogens elimination, damaged organelles and nutrient recycling to generate energy. Alterations in autophagic flux have been reported in the mechanism of several diseases such as neurodegenerative diseases, cancer, diabetes and its complications. Diabetic Retinopathy (DR) is a microvascular complication of diabetes, affecting nearly 30% of retinopathy. In the development of diabetic retinopathy, pathways are triggered or repressed and autophagy revealed to be relevant in this devastating complication. In this presentation, the role of autophagy in the development and progression of DR will be discussed, especially in pigmented epithelial, microvascular endothelial and muller cells, an important retinal glial cell.

Biography: Jacqueline M L Faria, MD, PhD completed her graduation at faculty of medical sciences, University of Campinas, SP, Brazil in 1990. After, she did residency in ophthalmology and post-doctoral fellow at Schepens Eye Research Institute, Harvard Medical School, Boston, MA. Back in Brazil, she finished the PhD program at University of Campinas. From 2000 and actually, has been senior scientist at Laboratory of Diabetic Microvascular Complications at faculty of medical sciences, University of Campinas, SP, Brazil and visiting professor at post-graduation course in the same University. Now, she is CEO of a nano-biotech startup focused in development of new treatments in ophthalmology (diabetic retinal diseases and related) for unmet medical needs. She coordinated 14 research projects, supervised 3 masters, 3 doctoral and 2 postdoctoral students; published 43 peer-reviewed papers in high impact International Scientific Journals with 970 times cited and 3 filled patents. She contributed with several reputed journals as independent reviewer.

2nd Global Webinar on Diabetes and Endocrinology 23

ORAL SPEAKERS

Evaluate the consumption of protein of animal-source in complementary foods of offspring of mothers with gestational diabetes in an educational intervention: 18-month follow-up Sandra Lorena Garcia Del Rio Instituto Mexicano del Seguro Social, Mexico

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e aimed to evaluate the consumption of protein of animal-source in the complementary diet of children of mothers with and without GD at 18 months of follow-up. A secondary analysis was performed in children participating in an ongoing study. Three groups were formed: with GD with intervention (GD+I), without GD with Intervention (woGD+I) and without GD without Intervention (woGD-I). The intervention consisted in monthly sessions promoting maternal lactation and orientation of complementary foods, in the first 3 months and trimonthly to month 18. The group woGD-I was given the standard information for the healthy child. The 3 groups answered 24-hour food recall and food frequency questionnaires (ff). For subanalysis, ff was analyzed with Chi2 at month 18. Adequate Consumption (AC) was analyzed with the combination of ff consumption per day per week and daily frequency. AC was defined: consuming animal-source food 1 day per week and >2 times per day or >2 days a week at least once a day. The sources that contain the most iron are liver and beef. Consumption of protein of animal origin with AC: Beef consumption in the GD+I was 83.1% and woGD+I 92.9% against 77.8% in the woGD-I group (p=0.023). Group woGD-I consumed liver more frequently than the group GD+I or woGD-I (60.7% and 47%, respectively, vs 55.5%, p=0.056). Group woGD+I consumed fish more frequently than groups GD+I and woGD-I (82.1% and 65%, respectively, vs 55.5%, p=0.053). GD+I was 98.8% and 100% in woGD+I vs 100% woGD-I (p