About our course Most physiological processes involve intracellular and cell-to-cell communication for their accomplishment. Therefore, it is not surprising that a great effort has been historically put into the study of secretion and membrane traffic and many drugs of clinical used are been developed thanks to the new knowledge acquired in this field. A major milestone and recognition for this field of science, has been the awarded Nobel Prize in 2013 to James Rothman, Randy Schekman and Thomas Südhof for their discoveries of machinery regulating vesicle traffic, a major transport system in our cells. In this III International Spring Course on “Measuring secretion and membrane traffic” we will combine talks given by recognized experts in the field with hands-on experiments on state of the art methods used to study membrane traffic and secretion. Students will have the opportunity to learn first-hand from some of the best experts in the field and always in reduced groups. We have made a great effort in combining good science and free time with one goal: students and senior scientists will share experiences in the outstanding environment of Alicante in spring and its charming surroundings. Welcome to Alicante! Luis Miguel Gutiérrez ([email protected]) Emilio Geijo ([email protected]) Ricardo Borges Guillermo Álvarez de Toledo

Professors Alés, Eva. Department of Physiology and Biophysics. University of Seville, Spain. Álvarez de Toledo, Guillermo. Department of Physiology and Biophysics. University of Seville, Spain. Barg, Sebastian. Medical Cell Biology. University of Uppsala. Sweden. Borges, Ricardo. Department of Physical Medicine and Pharmacology. University of La Laguna. Spain. Castellano-Muñoz, Manuel. Instituto de Bioingenieria. Universidad Miguel Hernández. Elche. Spain. Davletov, Bazbek. Department of Biomedical Science. University of Sheffield. UK. Ferrer-Montiel, Antonio. Instituto de Biologia Molecular y Celular. Universidad Miguel Hernández. Elche. Spain. García, Antonio G. Department of Pharmacology and Therapeutic. University Autónoma de Madrid, Spain. Geijo, Emilio. Instituto de Neurociencias. CSIC-Universidad Miguel Hernández. Alicante, Spain. Gutiérrez, Luis Miguel. Instituto de Neurociencias. CSIC-Universidad Miguel Hernández. Alicante, Spain. Lindau, Manfred. Department of Applied Physics and Engineering. Cornell University. USA. Seward, Elizabeth. Department of Biomedical Science. University of Sheffield. UK. Valdeolmillos, Miguel. Instituto de Neurociencias. CSIC-Universidad Miguel Hernández. Alicante, Spain.

Final Program

May 25, Wednesday

(Hotel Almirante, Playa de San Juan) 19.00-20:00 Registration. 20:00-20:10 Course presentation 20.15-21:00 Spanish Guitar concert by Riccardo Guella (Italy) and Kathy Acosta (Perú) from the International Master of Spanish Guitar (University of Alicante) 21:10-22:00 Opening Coctel

May 26, Thursday

(Hotel Almirante, Playa de San Juan) Chairman: Guillermo Alvarez de Toledo 09:00– SNAREv proteins and regulation of exocytosis Bazbek Davletov. University of Sheffield. UK. 09:40 – Pharmacological methods targeting the SNARE complex: from in silico to drug discovery Antonio Ferrer-Montiel. Instituto de Biología Molecular y Celular. Universidad Miguel Hernández. Alicante. Spain 10:20 – Experimental and Simulation Approaches to Reveal the Molecular Mechanisms of Fusion Pore Opening Manfred Lindau. Cornell University. USA 11:00-11.15 Coffe break 11:15 –Technical aspects of capacitance measurements. Guillermo Alvarez de Toledo . Universidad de Sevilla. Sevilla. Spain 11:55 –Dual sine capacitance: novel electrophysiological techniques in the study of exocytosis. Manuel Castellano-Muñoz. Instituto de Bioingenieria. Universidad Miguel Hernandez. Elche. Spain. 12:35 –Calcium signalling in developing neurons Miguel Valdeolmillos. Instituto de Neurociencias. CSIC-Universidad Miguel Hernández. Alicante, Spain. 13:05-13:30 Walk on sunshine. San Juan beach 13:30 Lunch at the Hotel 16: 00 – 20:00 Hands-on: (Laboratories of the Institute of Neurosciences) P1. F-actin cytoskeleton and chromaffin vesicles motion in confocal and TIRF microscopy. Luis M. Gutiérrez and José Villanueva. Instituto de Neurociencias. CSIC-UMH. Alicante. Spain.

P2. Measuring exocytosis using capacitance techniques Guillermo Alvarez de Toledo. Universidad de Sevilla. Sevilla. Spain and Manuel Castellano-Muñoz. Instituto de Bioingenieria. Universidad Miguel Hernandez. Elche. Spain. P3. Analysis of miniature postsynaptic currents Emilio Geijo. Instituto de Neurociencias. CSIC-Universidad Miguel Hernández. Alicante, Spain. P4. Calcium signals in neurons Miguel Valdeolmillos. Instituto de Neurociencias. CSIC-Universidad Miguel Hernández. Alicante, Spain. CSIC-Universidad Miguel Hernández. Alicante, Spain.

May 27, Friday (Hotel Almirante, Playa de San Juan) Chairman: Emilio Geijo 9:00 – Exocytotic fusion pore in neurodegenerative diseases Antonio G. Garcia. Universidad Autónoma de Madrid. Spain. 9:40 - Electrochemical methods for measuring secretion. Ricardo Borges. Universidad de La Laguna. Tenerife. Spain 10:20 – Using capacitance measurements to understand the regulation of stimulus-secretion coupling in adrenal chromaffin cells Liz Seward. University of Sheffield. UK.

11:00-11.15- Coffee break 11.15 – Imaging tools to study intracellular membrane traffic Sebastian Barg. University of Uppsala. Sweden 11.55 – Imaging and monitoring single granule recycling in mast cell Eva Alés.. Department of Physiology and Biophysics. University of Seville. Spain 12:35 –Confocal and TIRF microscopy studies to study F-actin implication in organelle traffic. Luis M. Gutiérrez. Instituto de Neurociencias. CSIC-Universidad Miguel Hernández. Alicante. Spain 13:05-13:30 Walk on sunshine. San Juan beach 13:30 Lunch at the Hotel 16: 00 – 20:00 Hands-on: (Laboratories of the Institute of Neurosciences) P1. F-actin cytoskeleton and chromaffin vesicles motion in confocal and TIRF microscopy. Luis M. Gutiérrez and José Villanueva. Instituto de Neurociencias. CSIC-UMH. Alicante. Spain. P2. Measuring exocytosis using capacitance techniques Guillermo Alvarez de Toledo. Universidad de Sevilla. Sevilla. Spain and Manuel Castellano-Muñoz. Instituto de Bioingenieria. Universidad Miguel Hernandez. Elche. Spain. P3. Analysis of miniature postsynaptic currents Emilio Geijo. Instituto de Neurociencias. CSIC-Universidad Miguel Hernández. Alicante, Spain.

P4. Calcium signals in neurons Miguel Valdeolmillos. Instituto de Neurociencias. CSIC-Universidad Miguel Hernández. Alicante, Spain.

21:00. Closing dinner in the harbour of Alicante.

May 28, Saturday 9:00 -16 :00 Excursion to the villages in the interior and the coast of Alicante province.

Registration Pre-registration is necessary since the course is limited to 25 students. For pre-registration send your personal data to the organizers ([email protected], [email protected]) before April 20th. If you are selected (mostly based in first arrive basis and your work orientation), you will be require to pay 300 euros by bank transfer (this includes registration, 3 nights hotel room in double room basis, and breakfast &meals). This low registration is possible thanks to the funding provided by different organizations. Course Venue and Hotel For your stay in Alicante: Hotel Almirante Playa de San Juan Avenida de Niza 38. Playa de San Juan. 03540. Alicante (Spain) http://www.hotelalmirante.com/

Morning sessions and Opening Ceremony will be at the hotel.

How to get from the Airport: Best option: Taxi fare about 35 euros.(30 min ride more or less) Cheapest option: Airport bus C6 to Alicante (Plaza del Mar, last station) and then take the Bus line 21 to the stop in the Avenida de la Costa Blanca/Maestro Barbieri), then a 6 min walk to the hotel. It will take you 1 h 30min. (about 5 euros)

Hands-on will be at: Instituto de Neurociencias de Alicante. CSIC-Universidad Miguel Hernández. Campus de San Juan. Cra de Valencia S/N. 03550. Alicante. http://in.umh.es/default.aspx

How to get from the Hotel. (We will provide a free bus). 3 Km (35 min walk).

List of Students

Carmen Martinez Ramírez. UAM. Madrid Fernanda Garcia Alvarado. UAM. Madrid Iago Mendez. UAM. Madrid Andrés Mateo Baraibar Sierra. UAM. Madrid Ayoze Gonzalez-Santana. ULL. Tenerife. Spain Michelle Juan Bandini. ULL. Tenerife. Spain Ahmed Alrifaiy. Gothenburg. Sweden. Ali Komai. Gothenburg. Sweden. Bassam Tawfik. Copenhange. Denmark Amr Eed. INA. Alicante Corinne Cobos Segura, INA. Alicante. Celia Román de la Calle. INA. Alicante. Laura Rincón Frutos. INA. Alicante. Aitana Sogorb. INA. Alicante. Yolanda Gimenez. INA. Alicante. Johanna Guadalupe Peña Del Castillo. UNAM. México

P1. Confocal and TIRF microscopy

P2. Capacitance techniques

May 26

Carmen Martinez

Ayoze GonzalezSantana

16-18 h

Fernanda Garcia Iago Mendez Andrés M Baraibar

P3.Miniature post-synaptic currents Bassam Tawfik. C

Laura Rincón Frutos.

18-20 h

Corinne Cobos

Ahmed Alrifaiy.

Celia Román de la Calle

Carmen Martinez

Aitana Sogorb.

Iago Mendez Yolanda Gimenez.

Johanna Guadalupe Peña Del Castillo.

Ayoze GonzalezSantana

Bassam Tawfik. C

Michelle Juan Bandini.

Amr Eed. Corinne Cobos

Andrés M Baraibar

Johanna Guadalupe Peña Del Castillo.

Ahmed Alrifaiy. Ali Komai.

Bassam Tawfik. C

Laura Rincón Frutos.

Amr Eed.

Aitana Sogorb.

16-18 h

Carmen Martinez

Celia Román de la Calle

Ayoze GonzalezSantana

Fernanda Garcia Iago Mendez Corinne Cobos Celia Román de la Calle

May 27

Yolanda Gimenez.

Fernanda Garcia Aitana Sogorb.

May 27

Laura Rincón Frutos.

Amr Eed. Michelle Juan Bandini.

Ali Komai.

May 26

P4. Calcium signals

Ayoze GonzalezSantana

18-20 h

Yolanda Gimenez.

Andrés M Baraibar

Johanna Guadalupe Peña Del Castillo.

Bassam Tawfik. C

Ahmed Alrifaiy. Ali Komai.

Ahmed Alrifaiy. Ali Komai.

Laura Rincón Frutos.

Amr Eed. Michelle Juan Bandini.

Michelle Juan Bandini.

Carmen Martinez Fernanda Garcia

Aitana Sogorb. Corinne Cobos Celia Román de la Calle

Iago Mendez Yolanda Gimenez. Johanna Guadalupe Peña Del Castillo.

Andrés M Baraibar

NOTES