OIE Reference Laboratory Reports Activities Activities in 2014
This report has been submitted : 2015-01-09 15:55:12
Name of disease (or topic) for which you are a designated OIE Reference Laboratory:
Leptospirosis
Address of laboratory:
SENASA Talcahuano N° 1660 Martínez, Pcia de Buenos Aires(1640) ARGENTINA INTA CICVyA Instituto de Patobiología Casilla de Correo 77 Morón 1708 Pcia de Buenos Aires ARGENTINA
Tel.:
+54 11 48361114 462
Fax:
+54 11 48361121 4521
E-mail address:
[email protected]
Website:
www.senasa.gov.ar www.inta.gov.ar
Name (including Title) of Head of Laboratory (Responsible Official):
Dr. Jorge Rodríguez Toledo Dr. Luis Samartino
Name (including Title and Position) of OIE Reference Expert:
MSc. Jessica Petrakovsky
[email protected] Dr Luis Samartino
[email protected]
Which of the following defines your laboratory? Check all that apply:
Governmental Other: Servicio Nacional de Sanidad y Calidad Agroalimentaria Instituto Nacional de Tecnologiía Agropecuaria
OIE Reference Laboratory Reports Activities, 2014
1
Leptospirosis - Jessica Petrakovsky - argentina
ToR 1: To use, promote and disseminate diagnostic methods validated according to OIE Standards
1. Did your laboratory perform diagnostic tests for the specified disease/topic for purposes such as disease diagnosis, screening of animals for export, surveillance, etc.? (Not for quality control, proficiency testing or staff training) Yes
Diagnostic Test
Indicated in OIE Manual (Yes/No)
Indirect diagnostic tests
Total number of test performed last year Nationally
Internationally 371
Microaglutinacion (MAT)
SI
3027
ELISA indirecta
SI
192
Direct diagnostic tests
Nationally
Aislamiento bacteriológico
SI
302
PCR
SI
88
Inmunofluorescencia directa
SI
208
Genotificación
102
Internationally
28
ToR 2: To develop reference material in accordance with OIE requirements, and implement and promote the application of OIE Standards. To store and distribute to national laboratories biological reference products and any other reagents used in the diagnosis and control of the designated pathogens or disease.
2. Did your laboratory produce or supply imported standard reference reagents officially recognised by the OIE? No 3. Did your laboratory supply standard reference reagents (non OIE-approved) and/or other diagnostic reagents to OIE Member Countries? Yes
2
OIE Reference Laboratory Reports Activities, 2014
Leptospirosis - Jessica Petrakovsky - argentina
Type of reagent available
Cepas patógenas
Cepas
Related diagnostic test
control de potencia de vacunas
MAT
Produced/ provide
160 ml
555
Amount supplied nationally (ml, mg)
120ml
340
Amount supplied internationally (ml, mg)
40 ml
50
No. of recipient OIE Member Countries
Region of recipients
2
Africa Americas Asia and Pacific Europe Middle East
3
Africa Americas Asia and Pacific Europe Middle East
4. Did your laboratory produce vaccines? No
5. Did your laboratory supply vaccines to OIE Member Countries? No
ToR 3: To develop, standardise and validate, according to OIE Standards, new procedures for diagnosis and control of the designated pathogens or diseases
6. Did your laboratory develop new diagnostic methods validated according to OIE Standards for the designated pathogen or disease? No 7. Did your laboratory develop new vaccines according to OIE Standards for the designated pathogen or disease? No
ToR 4: To provide diagnostic testing facilities, and, where appropriate, scientific and technical advice on disease control measures to OIE Member Countries
8. Did your laboratory carry out diagnostic testing for other OIE Member Countries? Yes
OIE Reference Laboratory Reports Activities, 2014
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Leptospirosis - Jessica Petrakovsky - argentina
Name of OIE Member Country seeking assistance
Date (month)
No. samples received for provision of diagnostic support
No. samples received for provision of confirmatory diagnoses
URUGUAY
Abril 2014
30
-
BRAZIL
Julio 2014
48
9. Did your laboratory provide expert advice in technical consultancies on the request of an OIE Member Country? Yes Name of the OIE Member Country receiving a technical consultancy
Purpose
How the advice was provided
URUGUAY
Control de Calidad de vacunas con Fraccion Leptospira
´Curso teórico - práctico en el SENASA
COLOMBIA
Manejo de Situaciones de brote de Leptospirosis
Mediante seminarios realizados en Colombia
CHILE
Diagnóstico de Leptospirosis en trabajadores de frigoríficos
Vía Correo electrónico
ARGENTINA
Consultas varias, brotes de leptospirosis, abortos, diagnostico serológico Presencial en el laboratorio,
visita a campo, "in situ"
ARGENTINA
Control de Calidad de vacunas con Fraccion Leptospira
Curso teórico - práctico en el SENASA
BRAZIL
Aislamiento y Tipificación molecular Diagnostico diferencial de leptospirosis
Asistencia a distancia, y visita "in situ
ECUADOR
Diagnostico de Leptospirosis por PCR Diagnostico diferencial de leptospirosis
Asistencia a distancia, y visita "in situ
URUGUAY
Aislamiento y Tipificación molecular
Asistencia a distancia
ToR 5: To carry out and/or coordinate scientific and technical studies in collaboration with other laboratories, centres or organisations
10. Did your laboratory participate in international scientific studies in collaboration with OIE Member Countries other than the own? Yes
4
OIE Reference Laboratory Reports Activities, 2014
Leptospirosis - Jessica Petrakovsky - argentina Title of the study
Duration
Purpose of the study
Partners (Institutions)
Tipificación de cepas
2 años
Diagnostico molecular de leptospirosis
Centro de Biotecnología, Universidad Federal de Pelotas
2 años
Diagnostico y tipificación molecular de leptospiras
Centro de Biotecnología, Universidad Federal de Pelotas Universidad Federal de San Pablo, Jaboticabao.
Desarrollo de un inmunoensayo para el diagnostico de leptospirosis en suinos
ToR 6: To collect, process, analyse, publish and disseminate epizootiological data relevant to the designated pathogens or diseases
11. Did your Laboratory collect epizootiological data relevant to international disease control? Yes 12. Did your laboratory disseminate epizootiological data that had been processed and analysed? Yes
13. What method of dissemination of information is most often used by your laboratory? (Indicate in the appropriate box the number by category) a) Articles published in peer-reviewed journals: 7 Animal Leptospirosis in Latin America and the Caribbean Countries. Jessica Petrakovsky, Alejandra Bianchi, Helen Fisun, Patricia Nájera-Aguilar, Martha M Pereira Int. J. Environ. Res. Public Health 2014, 11(10), 10770-10789. Basel, Switzerland. Octubre de 2014. -Sylvia Grune Loffler, Diego Passaro, Luis Samartino, Analía Soncini, Graciela Romero, Bibiana Brihuega. 2014. Genotypes of Leptospira spp. strains isolated from dogs in Buenos Aires, Argentina. Rev Argent Microbiol. 2014;46(3):201-204 -.Felipe Da Silva, Sylvia Grune, Bibiana Brihuega. 2014. Serological findings and isolation of Leptospira interrogans serovar Pomona in rural area of Maranhao, Brazil. Revista do Instituto de Medicina Tropical de Sao Paulo. -Mara Martínez, Sylvia Grune Loffler, Luis Samartino, Graciela Romero, Ignacio De la Fuente y Bibiana Brihuega. 2014. "Detección de leptospiras patógenas mediante PCR usando los iniciadores LigBF y LigBR". Rev Argentina de Zoonosis y Enfermedades Emergentes. Vol. IX Nº 2. junio 2014. -Grune Loffler S, Pavan ME, Vanasco B, Samartino L, Suarez O, Auteri C, Romero G, Brihuega B.2014. Genotypes of pathogenic Leptospira spp isolated from rodents in Argentina. Mem Inst Oswaldo Cruz. Rio de Janeiro: 1-5, 2014 - P. E. Martino,N. O. Stanchi, M. Silvestrini, B. Brihuega, L. Samartino,E. Parrado.2014. Seroprevalence for selected pathogens of zoonotic importance in wild nutria (Myocastor coypus). Eur J Wildl Res .March 2014. DOI 10.1007/s10344-014-0805-4 -Varni V, Ruybal P, Lauthier JJ, Tomasini N, Brihuega B, Koval A, Caimi K. 2014. Reassessment of MLST schemes for Leptospira spp. typing worldwide. Infect Gen Evol. Mar;22:216-22. b) International conferences: 4 Taller Internacional de Leptospirosis Humana y Animal. Conceptos Clásicos y Actualizaciones. Centro de
OIE Reference Laboratory Reports Activities, 2014
5
Leptospirosis - Jessica Petrakovsky - argentina Capacitación Buenos Aires para Servicios Veterinarios, CEBASEV. Buenos Aires, Argentina. Abril 2014. Coordinadora y Disertante “1er Encuentro Regional con Especialistas. Leptospira y Leptospirosis”. Auditorio SENA. Km1 - vía Acacias. Villavicencio. Colombia. Junio 2014 -Simposio de Leptospirosis. Coordinación y Disertación. III Congreso Panamericano de Zoonosis.VIII Congreso Argentino de Zoonosis.5 de junio de 2014, La Plata, Argentina. -Curso de Epidemiología y Diagnóstico de Leptospirosis. 10 al 12 de septiembre de 2014. Instituto de Patobiología. CICVyA-INTA Castelar. Buenos Aires, Argentina. Organizador.
c) National conferences: 5 Jornada de Actualización en Leptospirosis. Comisión Científica de Leptospirosis de la Asociación Argentina de Veterinarios de Laboratorios de Diagnóstico. Universidad Nacional de Tucumán. San Miguel de Tucumán, Noviembre de 2014. Disertante Jornada Causas infecciosas de Abortos en Caprinos de aborto. INTA Chamical La Rioja. 19 al 21 de agosto de 2014. Taller de Genotipificación de leptospira. 5 y 6 de septiembre de 2014. Instituto de Patobiología. CICVyA-INTA Castelar. Buenos Aires. Organizador. Curso-Taller de Ganadería Familiar: Formador de Formadores “Aspectos Sanitarios y Productivos en las diferentes especies” Disertación sobre diagnóstico y prevención de las zoonosis. Instituto de Patobiología. CICVyA-INTA Castelar, Buenos Aires. 5-.6 y 7 de noviembre de 2014. Disertación en el Curso CEBASEV. Enfermedades infecciosas de origen bacteriano. 2 al 4 de julio de 2014. Balcarce, Buenos Aires. d) Other: (Provide website address or link to appropriate information) 0
ToR 7: To provide scientific and technical training for personnel from OIE Member Countries To recommend the prescribed and alternative tests or vaccines as OIE Standards
14. Did your laboratory provide scientific and technical training to laboratory personnel from other OIE Member Countries? Yes a) Technical visits: 3 b) Seminars: 3 c) Hands-on training courses: 4 d) Internships (>1 month): 3
6
OIE Reference Laboratory Reports Activities, 2014
Leptospirosis - Jessica Petrakovsky - argentina Type of technical training provided (a, b, c or d)
Country of origin of the expert(s) provided with training
No. participants from the corresponding country
Visitas Técnicas
Uruguay, Colombia, Brasil
4, 50, 35
Seminarios
Uruguay, Paraguay, Chile, Colombia, Ecuador, Cuba, Italia
3, 2, 1, 3, 1, 2, 1
Formación práctica
Uruguay, Argentina, Brasil
4, 27, 1
Prácticas
Argentina, Uruguay, Brasil
5, 2, 2
ToR 8: To maintain a system of quality assurance, biosafety and biosecurity relevant for the pathogen and the disease concerned
15. Does your laboratory have a Quality Management System certified according to an International Standard? Yes
Quality management system adopted
Certificate scan (PDF, JPG, PNG format)
ISO/IEC 17025
Certificado 2012-2015.pdf
ISO/IEC 17025
Acreditacion 17025 Patobiologia MAT.pdf
16. Is your laboratory accredited by an international accreditation body? Yes Test for which your laboratory is accredited
Accreditation body
Microaglutinacion
Organismo Argentino de Acreditación (OAA)
17. Does your laboratory maintain a “biorisk management system” for the pathogen and the disease concerned? Yes (See Manual of Diagnostic Tests and Vaccines for Terrestrial Animals 2014, Chapter 1.1.3a)
ToR 9: To organise and participate in scientific meetings on behalf of the OIE
18. Did your laboratory organise scientific meetings on behalf of the OIE? Yes
OIE Reference Laboratory Reports Activities, 2014
7
Leptospirosis - Jessica Petrakovsky - argentina National/ International
Title of event
Co-organiser
Date (mm/yy)
Location
No. Participants
Internacional
• Taller Internacional de Leptospirosis Humana y Animal. Conceptos Clásicos y Actualizaciones. Centro de Capacitación Buenos Aires para Servicios Veterinarios, CEBASEV. Buenos Aires, Argentina.
Centro Buenos Aires para la capacitación de los servicios Veterinarios para Centro Colaborador de la OIE para la Capacitación de los Servicios Veterinarios
Abril 2014
Buenos Aires, Argentina
33
Internacional
III Congreso Panamericano de Zoonosis. Congreso Argentino de Zoonosis
Asociaci{on Argentina de Zoonosis
Junio 2014
La Plata, argentina
800
Nacional
Curso CEBASEV. Enfermedades infecciosas de origen bacteriano
Centro Buenos Aires para la capacitación de los servicios Veterinarios para Centro Colaborador de la OIE para la Capacitación de los Servicios Veterinarios
Julio 2014
Balcarce, Buenos Aires
40
Nacional
Taller de Genotipificación de leptospira
INTA
Septiembre 2014
Castelar, Buenos Aires
6
19. Did your laboratory participate in scientific meetings on behalf of the OIE? Yes
8
OIE Reference Laboratory Reports Activities, 2014
Leptospirosis - Jessica Petrakovsky - argentina
Title of event
Date (mm/yy)
“1er Encuentro Regional con Especialistas. Leptospira y Leptospirosis”. Auditorio SENA. Km1 - vía Acacias. Villavicencio. Colombia.
Junio 2014.
Location
Role (speaker, presenting poster, short communications)
Title of the work presented
Villavicencio. Colombia.
Orador
Recomendaciones ante brotes de Leptospirosis en grandes animales
II Congreso de la Sociedad Iberoamericana de Epidemiología Veterinaria y Medicina Preventiva
Noviembre 2014
Buenos Aires, Argentina
poster
Sistemas de Vigilancia Local: Identificación de Reservorios de Leptospira spp. En la reserva Ecológica Costanera Sur. Resultados Preliminares
Jornada de Actualización en Leptospirosis. Comisión Científica de Leptospirosis de la Asociación Argentina de Veterinarios de Laboratorios de Diagnóstico.
Noviembre 2014
Tucumán Argentina
orador
Control de Calidad de vacunas con fraccion leptospira
Third Global Conference of OIE Reference Centres
Octubre 2014
Incheon, Korea
participante
Actividades del Laboratorio de Referencia y Mandatos de a OIE referidos
poster
Detección de leptospiras patógenas en sueros bovinos mediante una PCR Multiplex. Grune S., Martinez M., Romero G., Brihuega B.
poster
Detección de leptospiras patógenas mediante PCR usando los iniciadoresLIGBpetf y LIGBptr. Martinez M, Grune S, Samartino L., Romero G.,Brihuega B
poster
Seroreactividad a Leptospira spp. en mamíferos pelíferos de la Provincia de Buenos Aires. Grune, S; Martino, P.; Martínez, M.; Romero, G.; Samartino, L.; Brihuega.
Asociación Argentina de Veterinarios de Laboratorio de Diagnóstico.
Asociación Argentina de Veterinarios de Laboratorio de Diagnóstico.
II Congreso de la Sociedad Iberoamericana de Epidemiología Veterinaria y Medicina Preventiva
Noviembre 2014
Noviembre 2014
Noviembre 2014
Tucumán, Argentina
Tucumán, Argentina
Buenos Aires, Argentina
OIE Reference Laboratory Reports Activities, 2014
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Leptospirosis - Jessica Petrakovsky - argentina
3rd Prato Conference of the Pathogenesis on Bacterial Diseases of Animals
III Congreso Panamericano de Zoonosis. Congreso Argentino de Zoonosis
Octubre 2014
Junio 2014
III Congreso Panamericano de Zoonosis. Congreso Argentino de Zoonosis
Junio 2014
VI. Congreso Virtual Iberoamericano sobre gestión de calidad en laboratorios-
Mayo a Noviembre 2014
Prato, Italia
La Plata, Argentina
La Plata, Argentina
On line
orador
Pathogenesis of abortion by leptospirosis. Bibiana Brihuega, Agustin Venzano, Sylvia Grune, Mara Martinez, Graciela Romero, Luis Samartino
poster
Estudio serológico de leptospirosis porcina en la llanura pampeana de la República Argentina. Mara Martínez, Sylvia Grune Loffler, Luis , Bibiana Brihuega.
poster
Coincidencia de perfiles genéticos de Leptospira borgpetersenii en animales silvestres y domésticos en Argentina. Sylvia Grune, Mara Martínez, Luis Samartino, Graciela Romero, Bibiana Brihuega
participante
Estudio de leptospirosis bovina bajo normas de calidad. Brihuega B, Schust M, Martinez M,Grune S., Romero S, Samartino L..
ToR 10: To establish and maintain a network with other OIE Reference Laboratories designated for the same pathogen or disease and organise regular inter-laboratory proficiency testing to ensure comparability of results
20. Did your laboratory exchange information with other OIE Reference Laboratories designated for the same pathogen or disease? Yes 21. Was your laboratory involved in maintaining a network with OIE Reference Laboratories designated for the same pathogen or disease by organising or participating in proficiency tests? Yes
1
10
Purpose of the proficiency tests: 1
Role of your Reference Laboratory (organiser/ participant)
No. participants
Participating OIE Ref. Labs/ organising OIE Ref. Lab.
Prueba de proficiencia de MAT 2014
participante
202
Laboratorio de Hereford Inglaterra, Royal Tropical Institute Holanda
validation of a diagnostic protocol: specify the test; quality control of vaccines: specify the vaccine type, etc.
OIE Reference Laboratory Reports Activities, 2014
Leptospirosis - Jessica Petrakovsky - argentina 22. Did your laboratory collaborate with other OIE Reference Laboratories for the same disease on scientific research projects for the diagnosis or control of the pathogen of interest? No
ToR 11: To organise inter-laboratory proficiency testing with laboratories other than OIE Reference Laboratories for the same pathogens and diseases to ensure equivalence of results
23. Did your laboratory organise or participate in inter-laboratory proficiency tests with laboratories other than OIE Reference Laboratories for the same disease? Yes Note: See Interlaboratory test comparisons in: Laboratory Proficiency Testing at: http://www.oie.int/en/our-scientific-expertise/reference-laboratories/proficiency-testing see point 1.3
Purpose for inter-laboratory test comparisons1
No. participating laboratories
Prueba de Proficiencia MAT INTA
Region(s) of participating OIE Member Countries Africa Americas Asia and Pacific Europe Middle East
8
ToR 12: To place expert consultants at the disposal of the OIE
24. Did your laboratory place expert consultants at the disposal of the OIE? Yes
Kind of consultancy
Location
Subject (facultative)
Evaluación de trabajos científicos para la Revista Cientifica de la OIE
Argentina
Revisión de trabajo sobre epidemiología de la leptospirosis
Revisión del Capitulo Leptospirosis Manual de las Pruebas de Diagnóstico y de las Vacunas para los Animales Terrestres de la OIE
Argentina
Diagnóstico y Control de Calidad de Vacunas
25. Additional comments regarding your report: El SENASA realiza el control de calidad de todas las vacunas para la prevención de la leptospirosis producidas por
OIE Reference Laboratory Reports Activities, 2014
11
Leptospirosis - Jessica Petrakovsky - argentina laboratorios privados tanto en la República Argentina como en otros países y asesora sobre el control y calidad de las mismas a otros países de la región El INTA interviene en proyectos de investigación nacionales e internacionales en el tema leptospirosis y da apoyo técnico práctico a los servicios veterinarios que requieran colaboración en diagnostico serológico y bacteriológico.
12
OIE Reference Laboratory Reports Activities, 2014